Characterization of the interaction between the transcription factors human polyamine modulated factor (PMF-1) and NF-E2-related factor 2 (Nrf-2) in the transcriptional regulation of the spermidine/spermine N1-acetyltransferase (SSAT) gene
Characterization of the interaction between the transcription factors human polyamine modulated factor (PMF-1) and NF-E2-related factor 2 (Nrf-2) in the transcriptional regulation of the spermidine/spermine N1-acetyltransferase (SSAT) gene
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DOI：
10.1042/0264-6021:3550045
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发表时间：
2001-04-01
影响因子：
4.1
通讯作者：
Casero, RA
中科院分区：
文献类型：
--
作者：
Wang, YL;Devereux, W;Casero, RA
多胺和多胺类似物已被证明可以调节各种基因的转录。亚精胺/精胺N-1-乙酰转移酶（SSAT）通过至少两种反式作用转录因子NF-E2相关因子2（Nrf-2）和PMF-1（多胺调节因子-1）的相互作用进行转录调节。Nrf-2先前已被证明通过其C-末端亮氨酸拉链与小Maf蛋白家族其他成员的亮氨酸拉链区域之间的相互作用来调节其他基因的转录（术语“Maf”源自肌肉腱膜纤维肉瘤病毒）。在这里，它表明，Nrf-2和PMF-1之间的相互作用是通过Nrf-2的亮氨酸拉链区域和PMF-1的C-末端卷曲螺旋区域，不包含亮氨酸拉链的结合介导的。中断Nrf-2的亮氨酸拉链或PMF-1的卷曲螺旋区域的突变被证明改变这些因子相互作用的能力，因此它们调节SSAT基因转录的能力丧失。
Polyamines and polyamine analogues have been demonstrated to modulate the transcription of various genes. Spermidine/ spermine N-1-acetyltransferase (SSAT) is transcriptionally regulated through the interaction of at least two trans-acting transcription factors, NF-E2-related factor 2 (Nrf-2) and PMF-1 (polyamine modulated factor-1). Nrf-2 has previously been shown to regulate transcription of other genes through interactions between its C-terminal leucine zipper and the leucine-zipper region of other members of the small Maf protein family (the term 'Maf' is derived from musculoaponeurotic-fibrosarcoma virus). Here it is demonstrated that the interaction between Nrf-2 and PMF-1 is mediated through the binding of the leucine-zipper region of Nrf-2 and a C-terminal coiled-coil region of PMF-1 that does not contain a leucine zipper. Mutations that interrupt either the leucine zipper of Nrf-2 or the coiled-coil region of PMF-1 are demonstrated to alter the ability of these factors to interact, thus their ability to regulate the transcription of the SSAT gene is lost.