Transcriptomic profiling of major carbon and amino acid metabolism in the roots of Arabidopsis thaliana treated with various rhizotoxic ions
Transcriptomic profiling of major carbon and amino acid metabolism in the roots of Arabidopsis thaliana treated with various rhizotoxic ions
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DOI：
10.1111/j.1747-0765.2009.00440.x
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发表时间：
2010-02
影响因子：
2
通讯作者：
Cheng-Ri Zhao;Y. Sawaki;N. Sakurai;D. Shibata;H. Koyama
中科院分区：
文献类型：
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作者：
Cheng-Ri Zhao;Y. Sawaki;N. Sakurai;D. Shibata;H. Koyama
摘要代谢过程的改变是植物对各种胁迫条件的一种普遍的适应性反应，可能受到复杂的调控。为了了解拟南芥对根毒害处理的代谢反应，比较了铝(Al)、铜(Cu)、镉(Cd)离子和类似严重程度的氯化钠处理下的主要碳和氨基酸代谢途径的转录组图谱。所有的胁迫处理都诱导了编码合成海藻糖和多胺的酶的基因，以及以前被认为对各种胁迫的抗性至关重要的色氨酸合成酶。在每种胁迫上调的基因中，编码抗坏血酸和精胺合成关键酶的基因对镉和氯化钠有较高的特异性。与其他基因相比，铝处理对苹果酸酶和谷氨酸脱羧酶的主要异构体的上调更为特异；这些酶属于细胞pH调节途径，即生化pH稳态途径和γ-氨基丁酸分流途径。对这些具有较高铜专一性的基因的鉴定表明，铜处理增强了氨基酸降解和类似糖饥饿的症状。海藻糖合成途径中的途径分析解释了该途径的激活和所有应激源积累的海藻糖。这些代谢变化可能构成了拟南芥根对根毒害离子耐性机制的一部分。
Abstract Alteration of metabolic processes is a common adaptive response of plants to various stress conditions and is likely to be under complex regulatory control. To understand the metabolic responses to rhizotoxic treatments in Arabidopsis thaliana, transcriptome profiles of major carbon and amino acid metabolic pathways were compared among aluminum (Al), copper (Cu) and cadmium (Cd) ion and NaCl treatments with a similar level of severity. All stress treatments induced genes encoding enzymes for synthesizing trehalose and polyamine, as well as tryptophan-synthesizing enzymes previously identified as critical for resistance to various stresses. Genes encoding enzymes critical for ascorbic acid and spermine synthesis had higher specificity to Cd and NaCl among the genes upregulated by each stress. Major isoforms of malic enzymes and glutamate decarboxylases were more specifically upregulated by the Al treatment than were other genes; these enzymes belong to cellular pH-regulating pathways, namely the biochemical pH stat pathway and the γ-amino butyric acid shunt. Characterization of the grouped genes with higher Cu specificity indicated that amino acid degradation and sugar starvation-like symptoms were enhanced by Cu treatment. Pathway analysis in the trehalose synthesis pathway accounted for the activation of the pathway and for the accumulation of trehalose by all stressors. These metabolic alterations might form part of the tolerance mechanisms of Arabidopsis roots against rhizotoxic ions.