HEMOCYTE POPULATION-CHANGES DURING THE IMMUNE-RESPONSE OF AEDES-AEGYPTI TO INOCULATED MICROFILARIAE OF DIROFILARIA-IMMITIS
HEMOCYTE POPULATION-CHANGES DURING THE IMMUNE-RESPONSE OF AEDES-AEGYPTI TO INOCULATED MICROFILARIAE OF DIROFILARIA-IMMITIS
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DOI：
10.2307/3282948
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发表时间：
1989-02-01
影响因子：
1.3
通讯作者：
CHRISTENSEN, LA
中科院分区：
文献类型：
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作者：
CHRISTENSEN, BM;HUFF, BM;CHRISTENSEN, LA
超微结构和凝集素结合研究已证实，埃及伊蚊利物浦株对接种的恶丝虫微丝蚴（mff）的黑素包囊反应是血细胞介导的反应。使用血腔灌注技术测定来自 mff 接种（=免疫激活）、盐水接种和未接种的雌性埃及伊蚊的总血细胞计数。未接种疫苗的蚊子的血细胞总数在年轻的蚊子中明显更大，但当蚊子年龄超过 14 天时，没有发现显着变化。免疫激活蚊子的血细胞数量在接种后 1 至 3 天增加，并在接种后第 4 和 5 天减少。与接种生理盐水的对照相比，接种 mff 的埃及伊蚊中 1 至 4 天的血细胞数量显着升高。接种盐水的蚊子在感染后 1 至 5 天的总血细胞数量变化不大，其血细胞数量与未接种的同龄昆虫相似。涉及接种[3H]胸苷与mff或单独盐水的实验以及涉及施用秋水仙碱的研究表明，免疫激活的埃及伊蚊中血细胞数量的增加是循环血细胞有丝分裂的结果。
Ultrastructural and lectin-binding studies have established that the melanotic encapsulation reaction of Aedes aegypti Liverpool strain against inoculated Dirofilaria immitis microfilariae (mff) is a hemocyte-mediated reaction. Total hemocyte counts from mff-inoculated (=immune-activated), saline-inoculated, and uninoculated female A. aegypti were determined using a hemocoel perfusion technique. Total hemocyte populations in uninoculated mosquitoes were significantly larger in younger mosquitoes, but no significant change was noted as mosquitoes aged beyond 14 days. Hemocyte populations in immune-activated mosquitoes increased from 1 to 3 days postinoculation (PI) and decreased on days 4 and 5 PI. Hemocyte populations at 1 to 4 days PI were significantly elevated in mff-inoculated A. aegypti as compared with saline-inoculated controls. Saline-inoculated mosquitoes displayed little change in total hemocyte numbers from 1 to 5 days PI, and their hemocyte populations were similar to those seen in uninoculated insects of the same age. Experiments involving the inoculation of [3H]thymidine along with mff or saline alone and studies involving the administration of colchicine suggest that increased hemocyte populations in immune-activated A. aegypti are a result of mitotic division of circulating blood cells.