BioFlux™ 200 Microfluidic System to Study A. baumannii Biofilm Formation in a Dynamic Mode of Growth
BioFlux™ 200 Microfluidic System to Study A. baumannii Biofilm Formation in a Dynamic Mode of Growth
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DOI：
10.1007/978-1-4939-9118-1_16
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发表时间：
2019-01-01
期刊：
影响因子：
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通讯作者：
Nicol, Marion
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文献类型：
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作者：
Naudin, Bertrand;Heins, Anja;Nicol, Marion
鲍曼不动杆菌在各种表面形成生物膜的能力与其在医院环境中的持久性以及顽固性和慢性感染的出现有关。几乎没有化合物可用于根除鲍曼不动杆菌生物膜，而且大多数化合物都在静态条件下对其抗生物膜性能进行了测试。像BioFlux(TM)系统这样的微流控系统现在可以用来研究动态条件下鲍曼不动杆菌生物膜的形成。在这里，我们描述了利用该系统在动态模式下研究参考菌株鲍曼不动杆菌ATCC 17978的生物膜发育。我们展示了如何在这些生长条件下测试抗生素(最低抑菌浓度为0.5微克/毫升的粘菌素)的活性。
The ability of A. baumannii to develop biofilms on a wide range of surfaces can be associated to its persistence in hospital settings and the emergence of recalcitrant and chronic infections. Few compounds are available to eradicate A. baumannii biofilms, and most of them have been tested for their antibiofilm properties in static conditions. Microfluidics systems as BioFlux (TM) system are now available for studying A. baumannii biofilm formation in dynamic conditions. Here, we described the use of this system for studying the biofilm development of the reference strain A. baumannii ATCC 17978 in a dynamic mode. We showed how to test the activity of an antibiotic (colistin at the MIC concentration, 0.5 mu g/mL) in these conditions of growth.