Semi-wet peptide/protein array using supramolecular hydrogel
Semi-wet peptide/protein array using supramolecular hydrogel
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DOI：
10.1038/nmat1034
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发表时间：
2004-01-01
期刊：
影响因子：
41.2
通讯作者：
Hamachi, I
中科院分区：
文献类型：
--
作者：
Kiyonaka, S;Sada, K;Hamachi, I
蛋白质微阵列是一种重要的生物材料，用于快速、高通量地分析许多涉及蛋白质的生物事件。与DNA微阵列相比，由于蛋白质在常规干燥条件下的不稳定性，它还没有得到充分的建立。在这里，我们报道了一种新型的半湿多肽/蛋白质微阵列，它使用由糖基化氨基醋酸酯组成的超分子水凝胶。这种超分子水凝胶的自发凝胶性和两亲性已被应用于一种新型的与酶分析兼容的多肽/蛋白质凝胶阵列。凝胶基质中产生的水腔是酶的合适的半湿反应介质，而纤维的疏水结构域是监测反应的独特场所。这种阵列系统克服了传统蛋白质芯片的一些缺点，因此在药物研究和诊断方面具有潜在的应用前景。
The protein microarray is a crucial biomaterial for the rapid and high-throughput assay of many biological events where proteins are involved. In contrast to the DNA microarray, it has not been sufficiently established because of protein instability under the conventional dry conditions. Here we report a novel semi-wet peptide/protein microarray using a supramolecular hydrogel composed of glycosylated amino acetate. The spontaneous gel-formation and amphiphilic properties of this supramolecular hydrogel have been applied to a new type of peptide/protein gel array that is compatible with enzyme assays. Aqueous cavities created in the gel matrix are a suitable semi-wet reaction medium for enzymes, whereas the hydrophobic domains of the fibre are useful as a unique site for monitoring the reaction. This array system overcomes several drawbacks of conventional protein chips, and thus can have potential applications in pharmaceutical research and diagnosis.