4-Aminopyridine reduces chorda tympani nerve taste responses to potassium and alkali salts in rat.
4-Aminopyridine reduces chorda tympani nerve taste responses to potassium and alkali salts in rat.
复制标题
4-Aminopyridine 降低大鼠鼓索神经对钾和碱金属盐的味觉反应。
DOI：
10.1016/0006-8993(93)91648-c
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发表时间：
1993
期刊：
影响因子：
2.9
通讯作者：
Mistretta,CM
中科院分区：
文献类型：
--
作者：
Kim,M;Mistretta,CM
为了研究钾通道在钾盐味觉反应中的潜在作用，我们将 4-氨基吡啶 (4-AP) 应用于大鼠前舌，并记录鼓索神经对化学刺激的味觉反应。 4-aminopyridine 是一种药理学阻断剂，可降低神经和肌肉钾通道的钾电导。比较了对溶解在水中和 4-AP 中的刺激的神经反应总和。化学刺激包括 KCl、KBr、KH2PO4、CsCl、RbCl、NH4Cl、NaCl 和蔗糖的浓度范围。该阻滞剂将鼓索对 KCl 和其他钾盐的反应从 0.025 M 降低至 0.25 M。对铵盐、铷盐和铯盐的反应也降低了，其有效性顺序可以根据钾通道的已知离子选择性特性进行预测。对氯化钠和蔗糖的反应没有降低。其他通道阻滞剂，包括四乙基氯化铵 (TEA)、BaCl2 和奎尼丁，不会降低对 KCl 的反应。这是关于钾通道阻滞剂对外周神经味觉反应影响的第一份详细报告。结果与顶端味蕾细胞膜中钾通道在钾盐转导中的作用一致。
To study the potential role of potassium channels in the taste response to potassium salts, we applied 4-aminopyridine (4-AP) to the anterior rat tongue and recorded chorda tympani nerve taste responses to chemical stimuli. 4-aminopyridine is a pharmacological blocker that reduces potassium conductance through potassium channels in nerve and muscle. Summated neural responses to stimuli dissolved in water and in 4-AP were compared. Chemical stimuli included concentration ranges of KCl, KBr, KH2PO4, CsCl, RbCl, NH4Cl, NaCl and sucrose. The blocker reduced chorda tympani responses to KCl and other potassium salts, from 0.025 to 0.25 M. Responses to ammonium, rubidium and cesium salts also were reduced, in order of effectiveness that would be predicted from known ion selectivity properties of potassium channels. Responses to NaCl and sucrose were not reduced. Other channel blockers, including tetraethylammonium chloride (TEA), BaCl2and quinidine, did not reduce the response to KCl. These are the first detailed reports of effects of potassium channel blockers on the peripheral, neural taste response. The results are consistent with a role for potassium channels in apical taste bud cell membranes in transduction for potassium salts.