Coherent oscillations in membrane potential synchronize impulse bursts in central olfactory neurons of the crayfish.
Coherent oscillations in membrane potential synchronize impulse bursts in central olfactory neurons of the crayfish.
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膜电位的相干振荡使小龙虾中枢嗅觉神经元的脉冲爆发同步。
DOI：
10.1152/jn.1999.81.3.1231
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Wheeler,CJ
中科院分区：
文献类型：
--
作者：
MellonJr,D;Wheeler,CJ
膜电位的相干振荡使螯虾中枢嗅觉神经元的脉冲群同步。 克氏原螯虾（Procambarus clarkiii）中央嗅觉通路中的外侧前脑中间神经元（LPIs）位于外侧前脑内，接受来自嗅中脑投射神经元的直接输入。LPI表现出周期性（0.5 Hz）的膜电位变化，施加在他们的突触。急性手术实验表明，突触活动起源于外侧前脑内的一组振荡神经元。来自许多LPI对的同时细胞内记录表明，这种周期性突触输入在大脑两侧的10200个LPI群体中是同步和连贯的。在许多LPI中，将特定的气味施加到分离的头部制备物中的触角上，产生持久的兴奋性突触后电位和脉冲群。脉冲群仅在持续去极化的峰值附近产生，在刺激应用后101 s，因此周期性基线活动有助于定时脉冲群的产生。同时记录从对LPI表明，当脉冲群发生在两个细胞后的气味刺激，它们是同步的共同的周期性去极化。我们的结论是，共同的，周期性的活动LPIs可以同步脉冲群在这些神经元的子集，可能会产生强大的持久的突触后效应在下游靶神经元。
Coherent oscillations in membrane potential synchronize impulse bursts in central olfactory neurons of the crayfish. Lateral protocerebral interneurons (LPIs) in the central olfactory pathway of the freshwater crayfishProcambarus clarkiireside within the lateral protocerebrum and receive direct input from projection neurons of the olfactory midbrain. The LPIs exhibit periodic (0.5 Hz) changes in membrane potential that are imposed on them synaptically. Acute surgical experiments indicate that the synaptic activity originates from a group of oscillatory neurons lying within the lateral protocerebrum. Simultaneous intracellular recordings from many LPI pairs indicate that this periodic synaptic input is synchronous and coherent among the population of ∼200 LPIs on each side of the brain. In many LPIs, specific odors applied to antennules in isolated head preparations generate long-lasting excitatory postsynaptic potentials and impulse bursts. The impulse bursts are generated only near the peaks of the ongoing depolarizations, ∼1 s after stimulus application, and so the periodic baseline activity is instrumental in timing burst generation. Simultaneous recordings from pairs of LPIs show that, when impulse bursts occur in both cells after an odorant stimulus, they are synchronized by the common periodic depolarizations. We conclude that the common, periodic activity in LPIs can synchronize impulse bursts in subsets of these neurons, possibly generating powerful long-lasting postsynaptic effects in downstream target neurons.