Depletion of arterial L-arginine causes reversible tolerance to endothelium-dependent relaxation.
Depletion of arterial L-arginine causes reversible tolerance to endothelium-dependent relaxation.
复制标题
动脉 L-精氨酸的消耗导致对内皮依赖性舒张的可逆耐受。
DOI：
10.1016/0006-291x(89)91518-0
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Ignarro,LJ
中科院分区：
文献类型：
--
作者：
Gold,ME;Bush,PA;Ignarro,LJ
本研究考察了l -精氨酸水平降低对牛肺动脉离体环内皮依赖性松弛的影响。动脉环在37°C的含氧碳酸氢盐溶液中张力培养24小时，导致对A23187（钙离子离子）和乙酰胆碱引起的松弛产生显著或完全耐受。对一氧化氮的松弛反应不受影响。添加l -精氨酸不会使控制环松弛，但会引起耐受环明显的内皮依赖性松弛，这种松弛被氧合血红蛋白或亚甲基蓝抑制。l -精氨酸也能恢复乙酰胆碱引起的松弛。在24小时的孵育中加入l -大麻碱可以防止耐受性的发展。耐受动脉环的组织精氨酸浓度比对照组低3倍，l -菜豆氨酸使动脉精氨酸水平恢复到控制值。因此，动脉l -精氨酸的消耗导致对内皮依赖性松弛的可逆性耐受。
This study examined the influence of lowered arterial levels of L-arginine on endothelium-dependent relaxation of isolated rings of bovine pulmonary artery. Incubation of arterial rings under tension for 24 hr in oxygenated Krebs bicarbonate solution at 37°C resulted in the development of marked or complete tolerance to A23187 (calcium ionophore)- and acetylcholine-elicited relaxation. Relaxant responses to nitric oxide were unaffected. Addition of L-arginine did not relax control rings but did elicit marked endothelium-dependent relaxation of tolerant rings that was inhibited by oxyhemoglobin or methylene blue. L-Arginine also restored acetylcholine-elicited relaxation. Inclusion of L-canavanine in the 24 hr incubations protected against the development of tolerance. The tissue concentration of arginine was 3-fold lower in tolerant than control arterial rings and L-canavanine restored arterial arginine levels to control values. Therefore, depletion of arterial L-arginine causes reversible tolerance to endothelium-dependent relaxation.