Widespread use of poly(A) tail length control to accentuate expression of the yeast transcriptome
Widespread use of poly(A) tail length control to accentuate expression of the yeast transcriptome
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DOI：
10.1261/rna.569407
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发表时间：
2007-07-01
期刊：
影响因子：
4.5
通讯作者：
Preiss, Thomas
中科院分区：
文献类型：
--
作者：
Beilharz, Traude H.;Preiss, Thomas
poly（A）尾长度的调控可以影响真核生物mRNA的翻译和稳定性。尽管在个别情况下已经得到证实，但尚不清楚这种类型的可调节基因控制在多大程度上用于塑造真核转录组的表达。在这里，我们报告了基于微阵列的mRNA poly（A）尾长度的测量以及与S中poly（A）结合蛋白Pab 1的相关性。酿酒酵母，揭示了广泛的相关性尾长和其他物理和功能的mRNA特征。基因本体分析和进一步的定向实验表明，尾长对功能和细胞定位相关mRNA的共调节，以协调细胞周期进程、核糖体生物合成和反转录转座子表达。我们发现3 '-非翻译区驱动转录特异性腺苷酸化控制和多种mRNA的翻译效率。我们的研究结果表明，芽殖酵母中3 '非翻译区介导的poly（A）尾长控制、Pab 1结合和mRNA翻译之间存在广泛的相互依赖关系。他们进一步提供了一个在细胞周期中的去腺苷酸酶功能的分子解释，并建议额外的细胞过程，依赖于mRNA聚腺苷酸化的控制。
Control of poly( A) tail length can affect translation and stability of eukaryotic mRNAs. Although well established for individual cases, it was not known to what extent this type of adjustable gene control is used to shape expression of eukaryotic transcriptomes. Here we report on microarray- based measurements of mRNA poly( A) tail lengths and association with the poly( A)- binding protein Pab1 in S. cerevisiae, revealing extensive correlation between tail length and other physical and functional mRNA characteristics. Gene ontology analyses and further directed experiments indicate coregulation of tail length on functionally and cytotopically related mRNAs to coordinate cell- cycle progression, ribosome biogenesis, and retrotransposon expression. We show that the 3'- untranslated region drives transcript- specific adenylation control and translational efficiency of multiple mRNAs. Our findings suggest a wide- spread interdependence between 3'- untranslated region- mediated poly( A) tail length control, Pab1 binding, and mRNA translation in budding yeast. They further provide a molecular explanation for deadenylase function in the cell cycle and suggest additional cellular processes that depend on control of mRNA polyadenylation.