Ex vivo pilot study using computed analysis of endo-cytoscopic images to differentiate normal and malignant squamous cell epithelia in the oesophagus
Ex vivo pilot study using computed analysis of endo-cytoscopic images to differentiate normal and malignant squamous cell epithelia in the oesophagus
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DOI：
10.1016/j.dld.2007.03.004
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发表时间：
2007-08-01
影响因子：
4.5
通讯作者：
Omata, M.
中科院分区：
文献类型：
--
作者：
Kodashima, S.;Fujishiro, M.;Omata, M.
背景和研究目的。细胞内镜系统允许采集与传统组织学非常相似的光学活检。为了简化使用细胞内镜系统在食管中正常和恶性组织之间的区分，我们分析了所获得的图像中的核（暗染色）区域，其目标是准确、自动诊断。使用10例入组患者的内镜或手术切除的食管进行离体细胞内镜观察。使用1%亚甲蓝对食管组织进行染色，并在每个食管的正常和恶性区域（各两个区域）获得细胞内镜图像。每幅图像的中心（4 × 10（-2）mm（2））由计算机处理，计算每个选定视野中总细胞核所占的面积及其占整个视野的比例。总细胞核的平均面积为0.10 x 10（-2）+/- 0.03 x 10（-2）mm（2）（范围0.05 x 10（-2）至0.18 x 10（-2）mm（2）），正常组为0.40 x 10（-2）+/- 0.06 x 10（-2）mm（2）恶性肿瘤组为0.33 × 10 ~（-2）~ 0.55 × 10 ~（-2）（P < 0.001）。正常组织中总细胞核与整个选定区域的平均比例为6.4 +/- 1.9%（范围3.1-11.3%），恶性样本中为25.3 +/- 3.8%（范围20.5-34.5%）（P < 0.001）。结论。细胞内窥镜系统可以自动区分食道正常组织和恶性组织，简化细胞内窥镜诊断。进一步的研究将阐明这种分析是否适用于食管或其他胃肠道器官中的炎症或癌前上皮。(C)2007年意大利胃肠病学编辑由爱思唯尔有限公司出版。保留所有权利。
Background and study aims. An endo-cytoscopy system allows acquisition of optical biopsies that are quite similar to conventional histology. To simplify discrimination between normal and malignant tissue in the oesophagus using endo-cytoscopy system, we analysed the nuclear (dark staining) area in the obtained images with the goal of an accurate, automatic diagnosis.Patients and methods. Ex vivo endo-cytoscopic observation was performed using endoscopically or surgically resected oesophagus from 10 enrolled patients. Oesophageal tissues were stained using 1% methylene blue, and endo-cytoscopic images were obtained at normal and malignant areas (two areas of each) in each oesophagus. The centre of each image (4 x 10(-2) mm(2)) was processed by computer, and the area occupied by the total nuclei in each selected field and its ratio to the entire field were calculated.Results. The mean area of the total nuclei was 0.10 x 10(-2) +/- 0.03 x 10(-2) mm(2) (range 0.05 x 10(-2) to 0.18 x 10(-2) mm(2) ) in the normal croup and 0.40 x 10(-2) +/- 0.06 x 10(-2) mm(2) (range 0.33 x 10(-2) to 0.55 x 10(-2)) in the malignant group (P < 0.001). The mean ratio of total nuclei to the entire selected field was 6.4 +/- 1.9% (range 3.1-11.3%) in the normal tissues and 25.3 +/- 3.8% (range 20.5-34.5%) in the malignant samples (P < 0.001).Conclusions. Endo-cytoscopy system allowed automatic differentiation of normal and malignant tissues in the oesophagus, which could simplify endo-cytoscopic diagnosis. Further study will elucidate whether such analysis is applicable to inflammatory or pre-malignant epithelia in the oesophagus or other gastrointestinal organs. (C) 2007 Editrice Gastroenterologica Italiana S.r.l. Published by Elsevier Ltd. All rights reserved.