Identification of Human Papillomavirus Type 58 Lineages and the Distribution Worldwide
Identification of Human Papillomavirus Type 58 Lineages and the Distribution Worldwide
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DOI：
10.1093/infdis/jir157
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发表时间：
2011-06-01
影响因子：
6.4
通讯作者：
Banks, Lawrence
中科院分区：
文献类型：
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作者：
Chan, Paul K. S.;Luk, Alfred C. S.;Banks, Lawrence
背景人乳头瘤病毒58型（HPV-58）在东亚宫颈癌中所占的比例远远高于其他类型。HPV-58的分类系统，这是必要的分子流行病学研究，是缺乏的。本研究分析了来自15个国家和城市的401株分离株的序列。占整个HPV-58基因组57%的268个独特的串联E6-E7-E2-E5-L1-LCR序列显示出4个不同的簇。L1和LCR产生的树状拓扑结构最类似于串联序列，因此是谱系分类的最合适的替代区域。此外，发现来自L1（核苷酸6014-6539）和LCR（核苷酸7257-7429和7540-52）的短片段含有为谱系鉴定提供信息的序列特征。A系是各大域最普遍的一系。谱系C在非洲比其他地方更常见，而谱系D在非洲比亚洲更普遍。在谱系A变体中，亚谱系A2在非洲、美洲和欧洲占主导地位，但在亚洲却没有。A1亚系代表起源于癌症患者的原型，除亚洲外，在世界范围内罕见。HPV-58可分为4个谱系，在分布上显示出一定程度的民族地理偏好。这些谱系的进化、流行病学和病理学特征值得进一步研究。
Background. Human papillomavirus type 58 (HPV-58) accounts for a much higher proportion of cervical cancers in East Asia than other types. A classification system of HPV-58, which is essential for molecular epidemiological study, is lacking.Methods and results. This study analyzed the sequences of 401 isolates collected from 15 countries and cities. The 268 unique concatenated E6-E7-E2-E5-L1-LCR sequences that comprised 57% of the whole HPV-58 genome showed 4 distinct clusters. L1 and LCR produced tree topologies that best resembled the concatenated sequences and thus are the most appropriate surrogate regions for lineage classification. Moreover, short fragments from L1 (nucleotides 6014-6539) and LCR (nucleotides 7257-7429 and 7540-52) were found to contain sequence signatures informative for lineage identification. Lineage A was the most prevalent lineage across all regions. Lineage C was more frequent in Africa than elsewhere, whereas lineage D was more prevalent in Africa than in Asia. Among lineage A variants, sublineage A2 dominated in Africa, the Americas, and Europe, but not in Asia. Sublineage A1, which represents the prototype that originated from a patient with cancer, was rare worldwide except in Asia.Conclusions. HPV-58 can be classified into 4 lineages that show some degree of ethnogeographic predilection in distribution. The evolutionary, epidemiological, and pathological characteristics of these lineages warrant further study.