Effect of heparin-binding growth factors on monovalent cation transport in Balb/C 3T3 cells.
Effect of heparin-binding growth factors on monovalent cation transport in Balb/C 3T3 cells.
复制标题
肝素结合生长因子对 Balb/C 3T3 细胞中单价阳离子转运的影响。
DOI：
10.1016/s0006-291x(87)80483-7
复制
发表时间：
1987
影响因子：
3.1
通讯作者：
Lobb,RR
中科院分区：
文献类型：
--
作者：
Halperin,JA;Lobb,RR
用Balb/C3 T3细胞研究了从牛脑中纯化的肝素结合生长因子1类和2类诱导的单价阳离子通量的变化。两类HBGF以浓度依赖性方式快速刺激Na+-K泵的活性，这与它们的促有丝分裂活性平行。这种作用需要外部Na+的存在，并被1 mM阿米洛利消除。与其他生长因子一样，HBGF通过阿米洛利敏感性Na+-H+交换增加Na+流入细胞，从而增加Na+-K+泵的活性。这些结果表明，阳离子转运的快速激活是HBGF与靶细胞表面特异性受体结合后的早期细胞反应。
The changes in monovalent cation fluxes induced by class 1 and class 2 heparin-binding growth factors purified from bovine brain were studied in confluent quiescent Balb/C 3T3 cells. Both classes of HBGF's rapidly stimulate the activity of the Na+-K pump in a concentration-dependent manner that parallels their mitogenic activity. This effect requires the presence of external Na+and is abolished by 1 mM amiloride. As with other growth factors, HBGF's increase the activity of the Na+-K+pump by increasing Na+influx into the cells via the amiloride-sensitive Na+-H+exchange. These results indicate that rapid activation of cation transport is an early cellular response that follows binding of HBGF's to specific receptors on the surface of target cells.