A novel spliced variant of the epithelial Na+ channel delta-subunit in the human brain.
A novel spliced variant of the epithelial Na+ channel delta-subunit in the human brain.
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DOI：
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发表时间：
2006
影响因子：
3.1
通讯作者：
H. Yamamura;S. Ugawa;T. Ueda;M. Nagao;S. Shimada
中科院分区：
文献类型：
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作者：
H. Yamamura;S. Ugawa;T. Ueda;M. Nagao;S. Shimada
阿米洛利敏感的上皮Na+通道调节细胞中的Na+稳态。最近，我们描述了δ-亚基是人脑中pH传感器的候选分子。在此，从人脑中克隆了δ-亚基的N-末端剪接变体，并将其命名为δ 2-亚基，其与原始δ-亚基（δ 1-亚基）在人脑中以相同水平表达。功能分析表明，δ 2-亚基的生理和药理学特性（与辅助β-γ亚基的相互作用，酸性pH值激活，阿米洛利敏感性）与δ 1-亚基的相似。总之，这两个亚基的活动可能参与了人类大脑中pH传感的机制。
The amiloride-sensitive epithelial Na+ channel regulates Na+ homeostasis in cells. Recently, we described that the delta-subunit is a candidate molecule for a pH sensor in the human brain. Here, an N-terminal spliced variant of the delta-subunit is cloned from human brain, and designated as the delta2-subunit, which is expressed with the original delta-subunit (delta1-subunit) at the same level in the human brain. Functional analyses revealed that the physiological and pharmacological properties (interaction with accessory betagamma-subunits, activation by acidic pH, amiloride sensitivity) of the delta2-subunit were similar to those of the delta1-subunit. In conclusion, the activities of both subunits may be involved in the mechanism underlying pH sensing in the human brain.