Infectious positive- and negative-strand transcript RNAs from bacteriophage Q beta cDNA clones.
Infectious positive- and negative-strand transcript RNAs from bacteriophage Q beta cDNA clones.
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来自噬菌体 Q beta cDNA 克隆的感染性正链和负链转录 RNA。
DOI：
10.1016/0042-6822(88)90250-4
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发表时间：
1988
期刊：
影响因子：
3.7
通讯作者：
Kaesberg,P
中科院分区：
文献类型：
--
作者：
Shaklee,PN;Miglietta,JJ;Palmenberg,AC;Kaesberg,P
构建了含有Qβ RNA噬菌体基因组全长cDNA拷贝和侧翼T7启动子的质粒。这些质粒通过T7 RNA聚合酶体外转录产生的正链Qβ RNA对大肠杆菌球质体具有传染性。从克隆的DNA中亚克隆了Oft复制酶基因并表达了inE。通过热诱导质粒培养结肠细胞。全长负链Qβ转录本转染到含有诱导复制酶基因的球质体中具有传染性。
Plasmids containing full-length cDNA copies of the Qβ RNA phage genome and flanking T7 promoters were constructed. Positive-strand Qβ RNA, generated byin vitrotranscription of these plasmids with T7 RNA polymerase, was infectious to Escherichia coli spheroplasts. The Oft replicase gene from the cloned DNA was subcloned and expressed inE. colicells by means of a thermoinducible plasmid. Full-length, negative-strand Qβ transcripts were infectious when transfected into spheroplasts containing the induced replicase gene.