Nanozyme and aptamer- based immunosorbent assay for aflatoxin B1
Nanozyme and aptamer- based immunosorbent assay for aflatoxin B1
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基于纳米酶和适体的黄曲霉毒素 B1 免疫吸附测定
DOI：
10.1016/j.jhazmat.2020.123154
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发表时间：
2020-11-15
影响因子：
13.6
通讯作者：
Liu, Jingmin
中科院分区：
文献类型：
--
作者：
Wu, Long;Zhou, Min;Liu, Jingmin
传统的酶联免疫吸附试验（ELISA）存在灵敏度低、稳定性差、酶标抗体制备和纯化困难等问题。基于纳米酶、核酸适体和Fe 3 O 4磁性纳米粒子（MNP），建立了一种检测黄曲霉毒素B1（AFB 1）的纳米酶-核酸适体免疫吸附法（NAISA）。本工作制备了介孔SiO2/Au-Pt（m-SAP）作为信号分子标记物，该分子标记物表现出较高的过氧化氢酶样活性，被称为纳米酶。采用核酸适体对黄曲霉毒素B1进行特异性识别，MNP辅助实现磁分离。为了验证NAISA的性能，将传统ELISA（t-ELISA）和使用MNP和m-SAP纳米酶的增强ELISA（e-ELISA）应用于AFB 1检测。NAISA方法的最低检测限（LOD）为5 pg mL（-1）（n = 3，+/-4.2%），分别比t-ELISA（3 ng mL（-1））和e-ELISA（0.06 ng mL（-1））低600和12倍。在干扰试验中，黄曲霉毒素B1可在6种不同的干扰物质中鉴别出来。因此，NAISA方法非常重要，因为它允许选择性和灵敏的AFB 1检测，同时提供了使用的简单性和筛选有害物质的需要。
Traditional enzyme-linked immunosorbent assay (ELISA) suffers from the limitations of relatively low sensitivity and stability, and enzyme-labelled antibodies are hard to be prepared and purified. Based on a nanozyme, an aptamer and Fe3O4 magnetic nanoparticles (MNP), a nanozyme and aptamer-based immunosorbent assay (NAISA) was developed for aflatoxin B1 (AFB1) detection with simpler operation and separation. In this work, mesoporous SiO2/Au-Pt (m-SAP) were prepared to act as signal labels, which showed high catalase-like activity and was denoted as nanozyme. Aptamer was adopted to specifically recognize with AFB1, and MNP facilitated to realize magnetic separation. To verify the performance of NAISA, traditional ELISA (t-ELISA) and enhanced ELISA (e-ELISA) using MNP and m-SAP nanozyme were applied in AFB1 detection. The NAISA method showed the lowest limit of detection (LOD) with 5 pg mL(-1) (n = 3, +/- 4.2 %), 600 and 12-fold lower than that of t-ELISA (3 ng mL(-1)) and e-ELISA (0.06 ng mL(-1)), respectively. In the interference tests, AFB1 can be identified among six different interfering substances. The NAISA method, thus, can be of great importance as it allows selective and sensitive AFB1 detection, while providing the simplicity of use and need for screening hazardous materials.