Testosterone increases and estradiol decreases middle cerebral artery occlusion lesion size in male rats
Testosterone increases and estradiol decreases middle cerebral artery occlusion lesion size in male rats
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DOI：
10.1016/s0006-8993(98)00327-8
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发表时间：
1998-06-15
期刊：
影响因子：
2.9
通讯作者：
Simpkins, JW
中科院分区：
文献类型：
--
作者：
Hawk, T;Zhang, YQ;Simpkins, JW
本研究旨在探讨雌激素和睾酮对雄性大鼠大脑中动脉（MCA）闭塞所致脑缺血损伤范围的影响。切除大鼠性腺，并用睾酮、雌激素或睾酮加雌激素填充的Silastic（R）丸处理。将动物分为6组：完整、完整+雌激素（E2）、去势、去势+睾酮（T）、去势+ E2和去势+ T + E2。治疗后1周，采用大脑中动脉阻断40 min，再灌注的方法制备脑缺血模型。24小时后，处死大鼠，然后将切片染色以评估病变大小。睾酮的存在增加和睾酮的去除减少病变大小。血浆睾酮浓度与缺血性病变大小呈强正相关（r（2）= 0.922）。雌二醇治疗减少了缺血面积。总之，本研究提供的证据表明，在脑缺血的动物模型中，睾酮加重缺血性脑损伤，而雌激素改善缺血性脑损伤。(C)1998 Elsevier Science B.V.保留所有权利。
This study was undertaken to determine the effects of estrogen and testosterone on cerebral ischemic lesion size induced by middle cerebral artery (MCA) occlusion in male rats. Rats were gonadectomized and treated with testosterone, estrogen, or testosterone plus estrogen filled Silastic(R) pellets. The animals were divided into 6 groups: intact, intact + estrogen (E2), castrate, castrate + testosterone (T), castrate + E2, and castrate + T + E2. One week after treatment, cerebral ischemia was induced by MCA occlusion for 40 min, followed by reperfusion. After 24 h, rats were sacrificed and slices were then stained to assess lesion size. The presence of testosterone increased and the removal of testosterone decreased lesion size. A strong positive correlation (r(2) = 0.922) between plasma testosterone concentrations and ischemic lesion size was observed. Estradiol treatment reduced ischemic area. In summary, the present study provides evidence that testosterone exacerbates and estrogens ameliorate ischemic brain damage in an animal model of cerebral ischemia. (C) 1998 Elsevier Science B.V. All rights reserved.