The mitogen-activated protein kinase p38 is involved in insect defense against Cry toxins from Bacillus thuringiensis.
The mitogen-activated protein kinase p38 is involved in insect defense against Cry toxins from Bacillus thuringiensis.
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DOI：
10.1016/j.ibmb.2009.12.010
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发表时间：
2010-01
影响因子：
3.8
通讯作者：
Bravo, Alejandra
中科院分区：
文献类型：
--
作者：
Cancino-Rodezno, Angeles;Alexander, Cynthia;Villasenor, Roberto;Pacheco, Sabino;Porta, Helena;Pauchet, Yannick;Soberon, Mario;Gill, Sarjeet S.;Bravo, Alejandra
关键词：
杀虫Cry毒素是由细菌苏云金芽孢杆菌（Bacillus thuringiensis）产生的破坏昆虫中肠细胞的成孔毒素。在这项工作中，我们分析了两种不同的昆虫目，鳞翅目天蛾和双翅目埃及伊蚊高度特异性的Cry毒素，Cry 1Ab和Cry 11 Aa，分别的反应。在不同生物体中响应于多种成孔毒素而激活的一种途径是激活复杂防御反应的促分裂原活化蛋白激酶p38途径（MAPK p38）。采用免疫组化方法检测MAPK p38的磷酸化水平，RT-PCR、真实的时间PCR定量分析和免疫组化方法检测MAPK p38的诱导表达。我们发现，MAPK p38激活后，在两个昆虫订单Cry毒素中毒后的postaductional水平。我们在转录和翻译水平检测到p38的诱导，并观察到不同的反应。在这三个水平上，我们发现这两种昆虫对Cry毒素的反应，但M sexta的反应比A。埃及人。体内MAPK p38的基因沉默导致两种昆虫物种对Cry毒素作用变得超敏感，表明MAPK p38途径参与昆虫对Bt Cry毒素的防御。这一发现可能具有增强某些Bt Cry毒素对特定昆虫害虫的活性的生物技术应用。
The insecticidal Cry toxins are pore-forming toxins produced by the bacteria Bacillus thuringiensis that disrupt insect-midgut cells. In this work we analyzed the response of two different insect orders, the Lepidopteran Manduca sexta and Dipteran Aedes aegypti to highly specific Cry toxins, Cry1Ab and Cry11Aa, respectively. One pathway activated in different organisms in response to a variety of pore forming toxins is the mitogen activated protein kinase p38 pathway (MAPK p38) that activates a complex defense response. We analyzed the MAPK p38 activation by immunodetection of its phosphorylated isoform, and the induction of p38 by RT-PCR, real time-PCR quantitative assays and immunodection. We show that MAPK p38 is activated at postraductional level after Cry toxin intoxication in both insect orders. We detected the p38 induction at the transcriptional and traductional level, and observed a different response. In these three levels, we found that both insects respond to Cry toxin action but M sexta responses more strongly than A. aegypti. Gene silencing of MAPK p38 in vivo, resulted in both insect species becoming hypersensitive to Cry toxin action, suggesting that the MAPK p38 pathway is involved in insect defense against Bt Cry toxins. This finding may have biotechnological applications for enhancing the activity of some Bt Cry toxins against specific insect pests.
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DOI：
10.1083/jcb.200708010
发表时间：
2008-03-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Idone V;Tam C;Goss JW;Toomre D;Pypaert M;Andrews NW
通讯作者：
Andrews NW
影响因子：
3.5
作者：
Husmann, Matthias;Beckmann, Erik;Bhakdi, Sucharit
通讯作者：
Bhakdi, Sucharit
影响因子：
64.5
作者：
Gurcel, Laure;Abrami, Laurence;van der Goot, F. Gisou
通讯作者：
van der Goot, F. Gisou
影响因子：
4.8
作者：
Ratner, AJ;Hippe, KR;Weiser, JN
通讯作者：
Weiser, JN
影响因子：
4.8
作者：
Jimenez-Juarez, Nuria;Munoz-Garay, Carlos;Bravo, Alejandra
通讯作者：
Bravo, Alejandra