SERUM IMMUNOGLOBULIN LEVELS AND NATURALLY-OCCURRING ANTIBODIES AGAINST CARBOHYDRATE ANTIGENS IN GERM-FREE BALB/C MICE FED CHEMICALLY DEFINED ULTRAFILTERED DIET
SERUM IMMUNOGLOBULIN LEVELS AND NATURALLY-OCCURRING ANTIBODIES AGAINST CARBOHYDRATE ANTIGENS IN GERM-FREE BALB/C MICE FED CHEMICALLY DEFINED ULTRAFILTERED DIET
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DOI：
10.1002/eji.1830191223
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发表时间：
1989-12-01
影响因子：
5.4
通讯作者：
MARCUS, DM
中科院分区：
文献类型：
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作者：
BOS, NA;KIMURA, H;MARCUS, DM
本研究探讨外源性抗原刺激对血清免疫球蛋白水平和针对碳水化合物抗原的循环天然抗体水平的影响。因此，使用在无菌环境中饲养并喂食化学成分超滤饮食（GF-CD）的 BALB/c 小鼠。这些小鼠的血清 IgM 水平正常，但 IgG 和 IgA 水平约为传统饲养同窝小鼠的 5%。所有四种 IgG 同种型的浓度同样低。针对多种碳水化合物抗原（包括 3-岩藻糖基乳糖胺 (3-FL)、左聚糖和葡聚糖）的天然 BALB/c 抗体的重链可变部分由 VH441 编码，这些抗体表达由单克隆抗体 6C4 和 6B1 识别的交叉反应性独特位。 GF-CD 中抗果聚糖和葡聚糖的抗体低于传统小鼠，但抗 3FL 抗体以及 6C4 和 6B1 独特位素的水平与传统动物相当。肽聚糖多糖复合物 (PPC) 是细菌来源的碳水化合物抗原，如果聚糖和半乳聚糖。在传统小鼠的血清中发现了天然存在的抗 PPC 抗体，但在 GF-CD 小鼠中却严重减少。结果表明，在常规小鼠中发现的大多数天然存在的针对细菌来源的碳水化合物抗原的抗体是由外源刺激引起的。
This study investigates the influence of exogenous antigenic stimulation on the serum immunoglobulin levels and the levels of circulating natural antibodies against carbohydrate antigens. Thus, BALB/c mice, raised in a germ-free environment and fed a chemically defined, ultrafiltered diet (GF-CD), were employed. These mice had normal serum IgM levels, but IgG and IgA levels were approximately 5% of conventionally reared littermates. The concentrations of all four IgG isotypes were equally low. The variable part of the heavy chains of naturally occurring BALB/c antibodies against a number of carbohydrate antigens, including 3-fucosyllactosamine (3-FL), levan and dextran, are encoded by VH441, and these antibodies express cross-reactive idiotopes recognized by the monoclonal antibodies 6C4 and 6B1. Antibodies against levan and dextran were lower in GF-CD than in conventional mice, but levels of anti-3FL antibodies, and 6C4 and 6B1 idiotopes, were comparable to those in conventional animals. Peptidoglycan polysaccharide complexes (PPC) are carbohydrate antigens of bacterial origin, like levan and galactan. Naturally occurring antibodies against PPC were found in the serum of conventional mice, but were severely reduced in GF-CD mice. The results indicate that most naturally occurring antibodies against carbohydrate antigens of bacterial origin found in conventional mice are caused by exogenous stimulation.