Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry.
Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry.
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通过使用电子捕获分离质谱法对蛋白质磷酸化的大规模定位。
DOI：
10.1074/mcp.m800451-mcp200
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发表时间：
2009-05
期刊：
影响因子：
--
通讯作者：
Cooper HJ
中科院分区：
文献类型：
--
作者：
Sweet SM;Bailey CM;Cunningham DL;Heath JK;Cooper HJ
我们使用在线电子捕获解离(ECD)来大规模鉴定和定位磷酸化位点。每个FT-ICR ECD事件都与一个线性离子陷阱碰撞诱导解离(CID)事件配对，从而可以直接比较ECD和CID在磷肽鉴定和位点定位方面的相对优点。线性离子陷阱CID对磷酸肽的鉴定最有效，而FT-ICR ECD在磷酸化位点的定位方面更具优势。在磷酸蛋白质组学实验中只鉴定一次磷酸肽的情况下，自信的CID和ECD鉴定以及自信的CID和ECD定位的组合特别有价值。
We used on-line electron capture dissociation (ECD) for the large scale identification and localization of sites of phosphorylation. Each FT-ICR ECD event was paired with a linear ion trap collision-induced dissociation (CID) event, allowing a direct comparison of the relative merits of ECD and CID for phosphopeptide identification and site localization. Linear ion trap CID was shown to be most efficient for phosphopeptide identification, whereas FT-ICR ECD was superior for localization of sites of phosphorylation. The combination of confident CID and ECD identification and confident CID and ECD localization is particularly valuable in cases where a phosphopeptide is identified just once within a phosphoproteomics experiment.