Satellite cells delivered by micro-patterned scaffolds: A new strategy for cell transplantation in muscle diseases
Satellite cells delivered by micro-patterned scaffolds: A new strategy for cell transplantation in muscle diseases
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DOI：
10.1089/ten.2006.0093
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发表时间：
2007-02-01
影响因子：
--
通讯作者：
De Coppi, Paolo
中科院分区：
文献类型：
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作者：
Boldrin, Luisa;Elvassore, Nicola;De Coppi, Paolo
成肌细胞移植是肌肉疾病的一种潜在有用的治疗工具，但缺乏有效的输送系统阻碍了其应用。在这里，我们将细胞生物学和聚合物加工相结合，为鼠卫星细胞（mSC）的体内移植创造合适的微环境。细胞由源自 C57BL/6-Tgn 增强型绿色荧光蛋白 (GFP) 转基因小鼠的单肌纤维制备。 mSC 被扩展并接种到使用软光刻和热膜层压制造的微图案聚乙醇酸 3 维支架中。然后使用免疫染色、流式细胞术和逆转录聚合酶链反应分析在体外评估肌原性。将含有 mSC 的支架植入 GFP 阴性同基因小鼠预损伤的胫骨前肌中。从培养皿中分离的细胞直接注射到对侧肢体作为对照。在这两种情况下，尽管植入支架的肌肉在 CD57 小鼠中显示出更多数量的 GFP 阳性纤维，但递送的细胞都参与了肌肉再生。这些发现表明，植入细胞支架比直接注射更能将生肌细胞输送到再生骨骼肌中。
Myoblast transplantation is a potentially useful therapeutic tool in muscle diseases, but the lack of an efficient delivery system has hampered its application. Here we have combined cell biology and polymer processing to create an appropriate microenvironment for in vivo transplantation of murine satellite cells (mSCs). Cells were prepared from single muscle fibers derived from C57BL/6-Tgn enhanced green fluorescent protein (GFP) transgenic mice. mSCs were expanded and seeded within micro-patterned polyglycolic acid 3-dimensional scaffolds fabricated using soft lithography and thermal membrane lamination. Myogenicity was then evaluated in vitro using immunostaining, flow cytometry, and reverse transcription polymerase chain reaction analyses. Scaffolds containing mSCs were implanted in pre-damaged tibialis anterior muscles of GFP-negative syngenic mice. Cells detached from culture dishes were directly injected into contra-lateral limbs as controls. In both cases, delivered cells participated in muscle regeneration, although scaffold-implanted muscles showed a much higher number of GFP-positive fibers in CD57 mice. These findings suggest that implantation of cellularized scaffolds is better than direct injection for delivering myogenic cells into regenerating skeletal muscle.