Bacteria-derived long chain fatty acid exhibits anti-inflammatory properties in colitis
Bacteria-derived long chain fatty acid exhibits anti-inflammatory properties in colitis
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DOI：
10.1136/gutjnl-2020-321173
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发表时间：
2021-06-01
期刊：
影响因子：
24.5
通讯作者：
Cenac, Nicolas
中科院分区：
文献类型：
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作者：
Pujo, Julien;Petitfils, Camille;Cenac, Nicolas
目的临床研究表明，某些益生菌菌株具有调节结肠炎症的潜力。尽管如此，这些数据不同的研究，由于益生菌菌株使用和知识贫乏，他们的mechanismofact.Design通过质谱，我们确定和定量的游离长链脂肪酸（LCFA）的益生菌和评估的影响，其中之一在小鼠colis.Results在所有LCFA量化的质谱在大肠杆菌Nissle 1917（EcN），作为一种用于治疗多种肠道疾病的益生菌，EcN中3-羟基十八碳烯酸（C18-3OH）的浓度比其他E.测试的大肠杆菌菌株。口服C18-3OH可降低葡聚糖硫酸钠诱导的小鼠结肠炎。为了确定构成微生物群的其他细菌是否能够产生C18-3OH，我们用益生元低聚果糖（FOS）靶向小鼠的肠道微生物群。FOS的抗炎特性与结肠C18-3OH浓度的增加相关。微生物群分析表明，C18-3OH的浓度与Allobaculum，Holdemanella和Parabacteroides中丰度的增加相关。在培养过程中，双形霍德曼氏菌产生高浓度的C18-3OH。最后，通过TR-FRET结合实验和基因表达分析，证实了C18-3OH是过氧化物酶体增殖物激活受体γ的激动剂。结论细菌产生C18-3OH可能是益生菌抗炎作用的机制之一。细菌产生LCFA-3OH可能与微生物群/宿主相互作用有关。
Objective Data from clinical research suggest that certain probiotic bacterial strains have the potential to modulate colonic inflammation. Nonetheless, these data differ between studies due to the probiotic bacterial strains used and the poor knowledge of their mechanisms of action.Design By mass-spectrometry, we identified and quantified free long chain fatty acids (LCFAs) in probiotics and assessed the effect of one of them in mouse colitis.Results Among all the LCFAs quantified by mass spectrometry in Escherichia coli Nissle 1917 (EcN), a probiotic used for the treatment of multiple intestinal disorders, the concentration of 3-hydroxyoctadecaenoic acid (C18-3OH) was increased in EcN compared with other E. coli strains tested. Oral administration of C18-3OH decreased colitis induced by dextran sulfate sodium in mice. To determine whether other bacteria composing the microbiota are able to produce C18-3OH, we targeted the gut microbiota of mice with prebiotic fructooligosaccharides (FOS). The anti-inflammatory properties of FOS were associated with an increase in colonic C18-3OH concentration. Microbiota analyses revealed that the concentration of C18-3OH was correlated with an increase in the abundance in Allobaculum, Holdemanella and Parabacteroides. In culture, Holdemanella biformis produced high concentration of C18-3OH. Finally, using TR-FRET binding assay and gene expression analysis, we demonstrated that the C18-3OH is an agonist of peroxisome proliferator activated receptor gamma.Conclusion The production of C18-3OH by bacteria could be one of the mechanisms implicated in the anti-inflammatory properties of probiotics. The production of LCFA-3OH by bacteria could be implicated in the microbiota/host interactions.