Subunits of the GPI transamidase complex localize to the endoplasmic reticulum and nuclear envelope in Drosophila
Subunits of the GPI transamidase complex localize to the endoplasmic reticulum and nuclear envelope in Drosophila
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GPI 转酰胺酶复合物的亚基定位于果蝇的内质网和核膜
DOI：
10.1002/1873-3468.14048
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发表时间：
2021
期刊：
影响因子：
3.5
通讯作者：
Goto Satoshi
中科院分区：
文献类型：
--
作者：
Kawaguchi Kohei;Yamamoto‐Hino Miki;Matsuyama Nina;Suzuki Emiko;Goto Satoshi
总共10-20%的质膜蛋白被糖基磷脂酰肌醇（GPI）锚定。GPI通过GPI转酰胺酶（GPI-T）连接到蛋白质上，GPI-T含有五个亚基，分别为PIGK、PIGS、PIGT、PIGU和GPAA 1。我们以前报道过PIGT定位于果蝇细胞核附近。然而，其他四个亚基的定位仍然未知。在这里，我们发现GPI-T的催化亚基PIGK主要定位于内质网（ER），而其他四个亚基定位于核膜（NE）和ER。不同细胞类型和不同发育阶段猪的NE/ER定位比例不同。我们的研究结果表明，GPI-T催化GPI在ER中的附着，其他四个亚基可能在NE中具有其他未知功能。
A total of 10–20% of plasma membrane proteins are anchored by glycosylphosphatidylinositol (GPI). GPI is attached to proteins by GPI transamidase (GPI‐T), which contains five subunits named PIGK, PIGS, PIGT, PIGU, and GPAA1. We previously reported that PIGT localizes near the nucleus inDrosophila. However, localizations of the other four subunits remain unknown. Here, we show that a catalytic subunit of GPI‐T, PIGK, mainly localizes to the endoplasmic reticulum (ER), while the other four subunits localize to the nuclear envelope (NE) and ER. The NE/ER localization ratio of PIGS differs between cell types and developmental stages. Our results suggest that GPI‐T catalyzes GPI attachment in the ER and the other four subunits may have other unknown functions in the NE.