Identification of a QTL conferring seedling and adult plant resistance to eyespot on chromosome 5A of Cappelle Desprez
Identification of a QTL conferring seedling and adult plant resistance to eyespot on chromosome 5A of Cappelle Desprez
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DOI：
10.1007/s00122-010-1427-1
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发表时间：
2011-01-01
影响因子：
5.4
通讯作者：
Nicholson, P.
中科院分区：
文献类型：
--
作者：
Burt, C.;Hollins, T. W.;Nicholson, P.
眼斑病是小麦和其他谷物的一种重要的经济真菌病害，由两种真菌引起：Oculimacula yallundae 和 Oculimacula acuformis。然而，只有两个眼斑抗性基因得到了表征，并且分子标记可供植物育种者使用。这些抗性是 Pch1（从近缘山羊草引入小麦）和 Pch2（最初在品种 Cappelle Desprez (CD) 中发现）。这两种电阻都存在缺点； Pch1 与 Ae 携带的有害性状有关。 ventricosa 基因渗入和 Pch2 已被证明效果有限。据报道，CD 5A 染色体上有一种额外的抗性，可赋予成年植物抗眼斑的能力。在本研究中，我们证明该染色体上的抗性对 O. yallundae 和 O. acuformis 眼斑病原体均有效，并在幼苗和成株阶段赋予抗性。这种抗性在幼苗生物测定和田间试验中都在由 CD 和携带来自易感品系 Bezostaya 的 5A 的 CD 单染色体替换品系之间杂交衍生的 5A 重组群体中进行了绘制。还利用幼苗生物测定对源自易感品系中国春(CS)和携带来自CD的5A的单染色体替代品系杂交的5A重组群体进行了抗性图谱绘制。在所有实验中均检测到 5A 染色体长臂上的一个主要 QTL。此外，SSR标记Xgwm639被发现与抗性密切相关，可用于植物育种者对眼斑抗性的标记辅助选择。
Eyespot is an economically important fungal disease of wheat and other cereals caused by two fungal species: Oculimacula yallundae and Oculimacula acuformis. However, only two eyespot resistance genes have been characterised and molecular markers made available to plant breeders. These resistances are Pch1, introduced into wheat from the relative Aegilops ventricosa, and Pch2, originally identified in the cultivar Cappelle Desprez (CD). There are drawbacks associated with both resistances; Pch1 is linked to deleterious traits carried on the Ae. ventricosa introgression and Pch2 has been shown to have limited effectiveness. An additional resistance has been reported on chromosome 5A of CD that confers resistance to eyespot in adult plants. In the present study, we demonstrate that resistance on this chromosome is effective against both O. yallundae and O. acuformis eyespot pathogens and confers resistance at both seedling and adult plant stages. This resistance was mapped in both seedling bioassays and field trials in a 5A recombinant population derived from a cross between CD and a CD single chromosome substitution line carrying 5A from the susceptible line Bezostaya. The resistance was also mapped using seedling bioassays in a 5A recombinant population derived from a cross between the susceptible line Chinese Spring (CS) and a single chromosome substitution line carrying 5A from CD. A single major QTL on the long arm of chromosome 5A was detected in all experiments. Furthermore, the SSR marker Xgwm639 was found to be closely associated with the resistance and could be used for marker-assisted selection of the eyespot resistance by plant breeders.