SEQUENCE AND EXPRESSION OF THE GLYCOSYL-PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C OF TRYPANOSOMA-BRUCEI
SEQUENCE AND EXPRESSION OF THE GLYCOSYL-PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE-C OF TRYPANOSOMA-BRUCEI
复制标题
DOI：
10.1016/0166-6851(89)90091-1
复制
发表时间：
1989-03-15
影响因子：
1.5
通讯作者：
OVERATH, P
中科院分区：
文献类型：
--
作者：
CARRINGTON, M;BULOW, R;OVERATH, P
布氏锥虫含有膜结合磷脂酶C，其将通过C-末端糖基-磷脂酰肌醇部分锚定在膜中的变体表面糖蛋白（BDG）转化为可溶性形式和二酰基甘油。该酶的氨基酸序列（358个残基）来源于cDNA和基因的核苷酸序列，揭示了一种多肽，其缺乏明显的N-末端信号序列和排他的疏水残基的延伸。这些性质表明磷脂酶在细胞质中合成，随后与细胞内膜结合或易位。血中糖基-磷脂酰肌醇特异性磷脂酶C mRNA的水平比原环型锥虫高得多。磷脂酶基因可能存在于一个或两个拷贝每个单倍体基因组，可能不与VSG表达位点。
Trypanosoma brucei contains a membrane-bound phospholipase C which converts the variant surface glycoprotein (BDG), anchored in the membrane by a C-terminal glycosyl-phosphtidylinositol moiety, into a soluble form and diacylglycerol. The amino acid sequence (358 residues) of this enzyme, derived form the nucleotide sequence of the cDNA and the gene, reveals a polypeptide which lacks an obvious N-terminal signal sequence and stretches of exclusively hydrophobic residues. These properties suggest that the phospholipase is synthesized in the cytoplasm and subsequently associates with or translocates across intracellular membranes. There are much higher levels of glycosyl-phosphatidyylinositol specific phospholipase C mRNA in bloodstream from than in procyclic form trypanosomes. The phospholipase gene is probably present in one or two copies per haploid genome, probably not associated with VSG expression sites.