iRoot BP Plus promotes osteo/odontogenic differentiation of bone marrow mesenchymal stem cells via MAPK pathways and autophagy
iRoot BP Plus promotes osteo/odontogenic differentiation of bone marrow mesenchymal stem cells via MAPK pathways and autophagy
复制标题
iRoot BP Plus 通过 MAPK 途径和自噬促进骨髓间充质干细胞的骨/牙源性分化
DOI：
10.1186/s13287-019-1345-3
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发表时间：
2019-07-29
影响因子：
7.5
通讯作者：
Yu, Jinhua
中科院分区：
文献类型：
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作者：
Lu, Jiamin;Li, Zehan;Yu, Jinhua
背景iRoot BP Plus 是一种新型生物陶瓷牙髓材料。最近，它被认为是 MTA 的替代品，MTA 是再生牙髓治疗期间最流行的支架覆盖物。本研究旨在评估iRoot BP Plus对骨髓间充质干细胞（BMMSC）成骨/成牙能力的影响，包括其潜在机制。方法采用全骨髓法收集BMMSC，并用iRoot BP Plus条件培养基（BP-CM）处理。采用细胞计数试剂盒8和流式细胞术评估增殖能力。以完全培养基作为空白对照，以2mg/ml MTA条件培养基作为阳性对照。通过碱性磷酸酶（ALP）活性测定、ALP染色、蛋白质印迹、实时RT-PCR、茜素红S染色和免疫荧光染色来探索成骨/成牙潜力以及MAPK通路的参与。此外，还通过蛋白质印迹、免疫荧光染色和透射电镜研究了自噬。 结果
BackgroundiRoot BP Plus is a novel bioceramic endodontic material. Recently, it has been considered as an alternative to MTA which is the most popular scaffold cover during regenerative endodontic therapy. This study aimed to evaluate the effects of iRoot BP Plus on the osteo/odontogenic capacity of bone marrow mesenchymal stem cells (BMMSCs), including the underlying mechanisms.MethodsBMMSCs were collected by a whole marrow method and treated with iRoot BP Plus-conditioned medium (BP-CM). The proliferation ability was evaluated by cell counting kit 8 and flow cytometry. Complete medium was used as a blank control, and 2mg/ml MTA-conditioned medium wasserved as a positive control. Alkaline phosphatase (ALP) activity assay, ALP staining, western blot, real-time RT-PCR, Alizarin Red S staining, and immunofluorescence staining were performed to explore the osteo/odontogenic potential and the involvement of MAPK pathways. Besides, autophagy was investigated by western blot, immunofluorescence staining, and transmission electron microscopy.Results