An isocratic assay for norepinephrine, dopamine, and 5-hydroxytryptamine using their native fluorescence by high-performance liquid chromatography with fluorescence detection in discrete brain areas of rat
An isocratic assay for norepinephrine, dopamine, and 5-hydroxytryptamine using their native fluorescence by high-performance liquid chromatography with fluorescence detection in discrete brain areas of rat
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DOI：
10.1006/abio.1996.9997
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发表时间：
1997-03-15
影响因子：
2.9
通讯作者：
Raju, TR
中科院分区：
文献类型：
--
作者：
Lakshmana, MK;Raju, TR
利用去甲肾上腺素(NE)、多巴胺(DA)和5-羟色胺(5-HT)的天然荧光，建立了高效液相色谱-荧光检测法同时测定去甲肾上腺素(NE)、多巴胺(DA)和5-羟色胺(5-HT)的方法。由于本方法不涉及样品的预纯化，单胺的回收率大于97%(n=12)，在给定的批次内，变异系数小于3.1%(n=12)。因此，使用内部标准不是强制性的。在单层析运行中，NE、DA和5-羟色胺的水平可在不到30分钟内测定。单胺类化合物的最低检测浓度NE和DA为250pg，5-羟色胺为100pg。用单胺氧化酶抑制剂Clorgyline处理后大鼠下丘脑和纹状体中单胺类物质含量的测定，证实了该方法的有效性。(C)1997年学术出版社。
A rapid and simple isocratic chromatographic procedure for simultaneous determination of norepinephrine (NE), dopamine (DA), and 5-hydroxytryptamin (5-HT) using their native fluorescence by high-performance liquid chromatography coupled to fluorescence detection (HPLC-FD) is described. Since the present procedure does not involve sample prepurification, the recovery of monoamines was more than 97% (n = 12) and within a given run, coefficient of variation was less than 3.1% (n = 12). Accordingly, use of an internal standard is not mandatory. In a single chromatographic run, levels of NE, DA, and 5-HT call be determined in less than 30 min. The minimum concentration of monoamines which could be detected by this method was found to be 250 pg for NE and DA and 100 pg for 5-HT. The validity of the method was confirmed by the estimation of levels of monoamines in the hypothalamus and striatum of rat brain following treatment with clorgyline, a monoamine oxidase inhibitor. (C) 1997 Academic Press.