Identification of genes up-regulated during conidiation of Fusarium oxysporum through expressed sequence tag analysis
Identification of genes up-regulated during conidiation of Fusarium oxysporum through expressed sequence tag analysis
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DOI：
10.1016/j.fgb.2005.11.003
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发表时间：
2006-03-01
影响因子：
3
通讯作者：
Tsuge, T
中科院分区：
文献类型：
--
作者：
Iida, Y;Ohara, T;Tsuge, T
尖镰孢产生三种无性孢子：小分生孢子、大分生孢子和厚垣孢子。尖孢镰刀菌在添加羧甲基纤维素的液体培养基（CMCLM）中产生小分生孢子和大分生孢子，并在完全液体培养基（CLM）中表现出无分生孢子的营养生长。使用来自 CLM 和 CMCLM 培养物的 mRNA 构建 cDNA 文库。分别对来自 CLM（营养生长）和 CMCLM（分生孢子）文库的总共 1288 个和 1353 个克隆进行了测序，并鉴定了 641 个和 626 个独特基因。在这些独特的基因中，只有 130 个（大约 20%）在两个文库中是常见的，表明营养生长和分生孢子形成过程中基因表达的模式不同。通过 cDNA 斑点印迹差异杂交和实时定量 PCR 分析，比较了营养生长和分生孢子形成过程中 496 个 CMCLM 特异性基因的表达水平，并鉴定出 42 个基因在分生孢子形成过程中 mRNA 丰度增加了 5 倍以上。这些基因为进一步研究了解真菌分生孢子发生及其分子调控提供了理想的候选基因。 (c) 2005 Elsevier Inc. 保留所有权利。
Fusarium oxysporum produces three kinds of asexual spores, microconidia, macroconidia, and chlamydospores. F oxysporum produces microconidia and macroconidia in carboxymethyl cellulose-added liquid medium (CMCLM) and exhibits vegetative growth without conidiation in complete liquid medium (CLM). The cDNA libraries were constructed using mRNAs from CLM and CMCLM cultures. A total of 1288 and 1353 clones from CLM (vegetative growth) and CMCLM (conidiation) libraries, respectively, were sequenced, and 641 and 626 unique genes were identified. Of these unique genes, only 130 (similar to 20%) were common in the two libraries, indicating different patterns of gene expression during vegetative growth and conidiation. The expression levels of 496 CMCLM-specific genes were compared during vegetative growth and conidiation by cDNA dot-blot differential hybridization and real-time quantitative PCR analyses, and 42 genes were identified to display > 5-fold increases in mRNA abundance during conidiation. These genes provide ideal candidates for further Studies directed at understanding fungal conidiogenesis and its molecular regulation. (c) 2005 Elsevier Inc. All rights reserved.