Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7
Transcribing RNA polymerase II is phosphorylated at CTD residue serine-7
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DOI：
10.1126/science.1145977
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发表时间：
2007-12-14
期刊：
影响因子：
56.9
通讯作者：
Eick, Dirk
中科院分区：
文献类型：
--
作者：
Chapman, Rob D.;Heidemann, Martin;Eick, Dirk
RNA聚合酶II的特征在于其大的羧基末端重复结构域（CTD），其由共有七肽Tyr（1）-Ser（2）-Pro（3）-Thr（4）-Ser（5）-Pro（6）-Ser（7）的重复组成。丝氨酸-2和丝氨酸-5在基因的5 '和3 '区域的差异磷酸化似乎协调转录和RNA加工因子到延伸聚合酶复合物的定位。使用单克隆抗体，我们揭示丝氨酸-7磷酸化转录基因。在少于20个共有重复序列的CTD中，该位置似乎未被磷酸化。丝氨酸-7被取代的重复序列的位置影响了不同磷酸化形式的出现，表明CTD区域之间的功能差异。我们的研究结果表明，限制丝氨酸-7表位的接头近端区域限制CTD磷酸化模式，是最佳的基因表达的要求。
RNA polymerase II is distinguished by its large carboxyl- terminal repeat domain ( CTD), composed of repeats of the consensus heptapeptide Tyr(1)-Ser(2)-Pro(3)-Thr(4)-Ser(5)-Pro(6)-Ser(7). Differential phosphorylation of serine-2 and serine-5 at the 5 ' and 3 ' regions of genes appears to coordinate the localization of transcription and RNA processing factors to the elongating polymerase complex. Using monoclonal antibodies, we reveal serine-7 phosphorylation on transcribed genes. This position does not appear to be phosphorylated in CTDs of less than 20 consensus repeats. The position of repeats where serine-7 is substituted influenced the appearance of distinct phosphorylated forms, suggesting functional differences between CTD regions. Our results indicate that restriction of serine-7 epitopes to the Linker- proximal region limits CTD phosphorylation patterns and is a requirement for optimal gene expression.