Development of a novel sticky trap for container-breeding mosquitoes and evaluation of its sampling properties to monitor urban populations of Aedes albopictus
Development of a novel sticky trap for container-breeding mosquitoes and evaluation of its sampling properties to monitor urban populations of Aedes albopictus
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DOI：
10.1111/j.1365-2915.2007.00680.x
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发表时间：
2007-06-01
影响因子：
1.9
通讯作者：
Della Torre, A.
中科院分区：
文献类型：
--
作者：
Facchinelli, L.;Valerio, L.;Della Torre, A.
目前用于大规模采样成年隐蚊（双翅目：蚊科）的采集方法存在一些操作局限性，这对虫媒病毒的流行病学监测、控制策略影响的评估以及外来物种向非流行地区的传播的监测构成了重大缺陷。在这里，我们描述了一种新的粘性陷阱，旨在捕获成年容器繁殖的蚊子并监测其种群动态。我们在意大利罗马测试了粘虫捕捉器的采样特性，白纹伊蚊在那里很常见。介绍了我们的观察结果，以及粘性诱捕器捕获物与标准产卵诱捕器捕获物之间的比较。粘性捕集器收集了明显大量的白粉病。雌性白纹伊蚊数量超过任何其他蚊科物种，占总渔获量的 90% 以上。最大 83 安。在一周内收集了雌性白纹伊蚊。雌性数量与诱捕器收集的卵数量之间存在高度相关性（皮尔逊相关系数 r = 0.96）。通过拟合 log(1 + x) 转换的陷阱计数（y = 0.065 + 1.695x）的主轴回归来评估卵数量和成年雌性数量之间的函数关系。陷阱样本明显偏离随机分布；将泰勒幂律拟合到诱捕器样本上，以量化捕获物的聚集程度，返回粘性诱捕器的方程 s(2) = 2.401 m(1.325) 和产卵器的 s(2) = 13.068 m(1.441)，其中 s(2) 和 m 分别表示每周捕获量方差和平均值，表明卵明显比蚊子更聚集（P < 0.0001）。泰勒幂律参数用于估计获得具有固定精度和灵敏度的样本估计值所需的最小样本单位数。对于 Ae 期间我们研究区域遇到的密度范围。白纹伊蚊繁殖季节，粘性诱捕器比产卵器更加精准、灵敏。然而，在种群密度较低时（c. < 0.1 只蚊子/诱捕器），产卵器在检测该物种的存在时更加敏感。总的来说，我们的结果表明我们的新模型粘性捕集器可用于对AE进行采样。城市环境中的雌性白纹伊蚊，以及可能的其他容器繁殖的隐蚊（例如埃及伊蚊）。讨论了新诱捕器的技术特性，讨论了其在监测容器繁殖蚊子的种群动态、研究其生物学、媒介监测和可能的控制方面的可能应用。
Collection methods currently used for large-scale sampling of adult Stegomyia mosquitoes (Diptera: Culicidae) present several operational limitations, which constitute major drawbacks to the epidemiological surveillance of arboviruses, the evaluation of the impact of control strategies, and the surveillance of the spreading of allochthonous species into non-endemic regions. Here, we describe a new sticky trap designed to capture adult container-breeding mosquitoes and to monitor their population dynamics. We tested the sampling properties of the sticky trap in Rome, Italy, where Aedes (Stegomyia) albopictus is common. The results of our observations, and the comparison between sticky trap catches and catches made with the standard oviposition trap, are presented. The sticky trap collected significantly larger numbers of Ae. albopictus females than any other Culicidae species representing > 90% of the total catches. A maximum of 83 An. albopictus females was collected in a single week. A high correlation (Pearson correlation coefficient r = 0.96) was found between the number of females and the number of eggs collected by the traps. The functional relationship between the number of eggs and the number of adult females was assessed by major axis regression fitted to log(1 + x)-transformed trap counts as y = 0.065 + 1.695x. Trap samples significantly departed from a random distribution; Taylor's power law was fitted to the trap samples to quantify the degree of aggregation in the catches, returning the equations s(2) = 2.401 m(1.325) for the sticky trap and s(2) = 13.068 m(1.441) for the ovitrap, with s(2) and m denoting the weekly catch variance and mean, respectively, indicating that eggs were significantly more aggregated than mosquitoes (P < 0.0001). Taylor's power law parameters were used to estimate the minimum number of sample units necessary to obtain sample estimates with a fixed degree of precision and sensitivity. For the range of densities encountered in our study area during the Ae. albopictus breeding season, the sticky trap was more precise and sensitive than the ovitrap. At low population densities (c. < 0.1 mosquito/trap), however, the ovitrap was more sensitive at detecting the presence of this species. Overall, our results indicate that our new model of sticky trap can be used to sample Ae. albopictus females in urban environments, and, possibly, other container-breeding Stegomyia mosquitoes (e.g. Aedes aegypti). The technical properties of the new trap are discussed with respect to its possible application in monitoring the population dynamics of container-breeding mosquitoes, in studying their bionomics, and in vector surveillance and, possibly, control.