Prognostic impact of β-2-microglobulin expression in colorectal cancers stratified by mismatch repair status
Prognostic impact of β-2-microglobulin expression in colorectal cancers stratified by mismatch repair status
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DOI：
10.1136/jclinpath-2012-200742
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发表时间：
2012-11-01
影响因子：
3.4
通讯作者：
Zlobec, Inti
中科院分区：
文献类型：
--
作者：
Koelzer, Viktor Hendrik;Baker, Kristi;Zlobec, Inti
背景β 2-微球蛋白（B2 M）是抗原提呈的必需物质，但也可能具有原癌基因的特性。目的探讨B2 M对错配修复（MMR）阳性和缺陷型结直肠癌（CRC）患者预后的影响，并探讨这种影响是否依赖于局部免疫应答。方法采用免疫组织化学方法检测B2 M蛋白表达和肿瘤浸润免疫细胞（PBMC），并与结直肠癌患者进行比较。结果B2 M蛋白表达与结直肠癌患者的局部免疫应答有关，而B2 M蛋白表达与结直肠癌患者的局部免疫应答有关。（CD 3、CD 16、CD 163、CD 20、CD 4、CD 45 RO、CD 56、CD 68、CD 8、FoxP 3、GranzymeB、iNOS、肥大细胞类胰蛋白酶、MUM 1、PD 1、TIA-1）。B2 M表达的临床病理特征的预测价值和预后意义进行了分析，分层的MMR状态和免疫细胞infiltration.Results的免疫组织学特征的B2 M染色的观察者之间的协议是高（组内相关系数=0.91）。MMR缺陷型（19.4%）的B2 M完全丢失率高于MMR正常型（7.1%）（p <0.05）。
Background beta-2-microglobulin (B2M) is essential for antigen presentation, yet may also possess proto-oncogenic properties.Aim To determine the prognostic impact of B2M in patients with mismatch repair (MMR) proficient and deficient colorectal cancer (CRC) and to investigate whether this effect on outcome is dependent on the local immune response.Methods B2M protein expression and tumour-infiltrating immune cells (CD3, CD16, CD163, CD20, CD4, CD45RO, CD56, CD68, CD8, FoxP3, GranzymeB, iNOS, mast cell tryptase, MUM1, PD1, TIA-1) were evaluated in a well characterised tissue microarray of 408 CRCs. The predictive value for clinicopathological features and the prognostic significance of B2M expression were analysed, stratified by MMR status and the immunohistological characteristics of immune cell infiltrates.Results Interobserver agreement for B2M staining was high (intra-class correlation coefficient=0.91). Complete B2M loss was more frequent in MMR-deficient (19.4%) compared to MMR-proficient (7.1%) tumours (p