Thermo-optical characterization of fluorescent rhodamine B based temperature-sensitive nanosensors using a CMOS MEMS micro-hotplate.
Thermo-optical characterization of fluorescent rhodamine B based temperature-sensitive nanosensors using a CMOS MEMS micro-hotplate.
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DOI：
10.1016/j.snb.2013.10.042
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发表时间：
2014-03-01
期刊：
影响因子：
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通讯作者：
Aylott JW
中科院分区：
文献类型：
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作者：
Chauhan VM;Hopper RH;Ali SZ;King EM;Udrea F;Oxley CH;Aylott JW
我们报告了一种定制设计的MEMS微热板的发展，能够在高温（高达700摄氏度）下工作。MEMS微热板可以直接安装在荧光共聚焦显微镜的载玻片保持器中。通过将罗丹明B共价连接到二氧化硅溶胶-凝胶基质上，合成了温度敏感的纳米传感器（直径550 nm）。使用MEMS装置和共聚焦显微镜对纳米传感器进行热光学表征。纳米传感器的温度依赖性荧光响应被发现在宽范围内工作，高达145摄氏度。定制设计的能够在高温（高达700 °C）下操作的微机电系统（MEMS）微热板用于热光学表征荧光温度敏感纳米传感器。纳米传感器，550 nm的直径，是由温度敏感的罗丹明B（Rh B）荧光团，这是共轭的惰性硅溶胶-凝胶基质。将温度敏感的纳米传感器分散并干燥在MEMS微热板的表面上，该MEMS微热板安装在荧光共聚焦显微镜的载玻片保持器中。通过对MEMS微热板的电气控制，在宽的温度范围内测量纳米传感器的荧光强度的温度诱导的变化。当温度从25 °C增加到145 °C时，发现分散在MEMS器件表面上的所有纳米传感器的荧光响应以指数方式降低94%。使用线轮廓分析，在MEMS装置的整个表面上的所有分散的纳米传感器和单个纳米传感器的荧光响应没有统计学差异（p < 0.05）。用于本研究的MEMS器件可以被证明是一种可靠的，低成本的，低功耗和高温微热板的亚微米尺寸的颗粒的热光特性。这种温度敏感纳米传感器在生物和微电子系统的温度测量中具有潜在的应用前景。
We report the development of a custom designed MEMS micro-hotplate capable of operating at high temperatures (up to 700 ̊C). The MEMS micro-hotplate can be directly mounted in a slide holder of a fluorescent confocal microscope. Temperature-sensitive nanosensors (550 nm diameter) were synthesised by covalently linking rhodamine B to a silica sol–gel matrix. Nanosensors were thermo-optically characterised using the MEMS device and confocal microscopy. The temperature dependent fluorescence response of the nanosensors was found to operate over wide range, up to 145 ̊C. A custom designed microelectromechanical systems (MEMS) micro-hotplate, capable of operating at high temperatures (up to 700 °C), was used to thermo-optically characterize fluorescent temperature-sensitive nanosensors. The nanosensors, 550 nm in diameter, are composed of temperature-sensitive rhodamine B (RhB) fluorophore which was conjugated to an inert silica sol–gel matrix. Temperature-sensitive nanosensors were dispersed and dried across the surface of the MEMS micro-hotplate, which was mounted in the slide holder of a fluorescence confocal microscope. Through electrical control of the MEMS micro-hotplate, temperature induced changes in fluorescence intensity of the nanosensors was measured over a wide temperature range. The fluorescence response of all nanosensors dispersed across the surface of the MEMS device was found to decrease in an exponential manner by 94%, when the temperature was increased from 25 °C to 145 °C. The fluorescence response of all dispersed nanosensors across the whole surface of the MEMS device and individual nanosensors, using line profile analysis, were not statistically different (p < 0.05). The MEMS device used for this study could prove to be a reliable, low cost, low power and high temperature micro-hotplate for the thermo-optical characterisation of sub-micron sized particles. The temperature-sensitive nanosensors could find potential application in the measurement of temperature in biological and micro-electrical systems.