Ganglioside composition and histology of a spontaneous metastatic brain tumour in the VM mouse
Ganglioside composition and histology of a spontaneous metastatic brain tumour in the VM mouse
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DOI：
10.1054/bjoc.2001.1909
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发表时间：
2001-07-20
影响因子：
8.8
通讯作者：
Lee, MC
中科院分区：
文献类型：
--
作者：
El-Abbadi, M;Seyfried, TN;Lee, MC
神经节苷脂是一类含有唾液酸的神经鞘糖脂，它调节细胞与细胞之间以及细胞与基质之间的相互作用。在VM小鼠品系的天然脑肿瘤中检查组织学和神经节苷脂组合物。该肿瘤与其他转移性肿瘤模型不同，因为其自发产生，并在侧腹皮下接种肿瘤组织后转移至几个器官，包括脑和脊髓。通过电子显微镜，肿瘤由细胞（直径15至20微米），有轻微的缩进核和细胞质不足。光滑膜的存在下，没有连接复合物是一个典型的超微结构特征。神经胶质或神经元标记物未见阳性免疫染色。皮下生长的肿瘤的总神经节苷脂唾液酸含量较低（12.6 +/- 0.9 μ g/100 mg干重，n = 6个单独的肿瘤），其中约70%以N-羟乙酰神经氨酸的形式存在。相反，培养的VM肿瘤细胞的神经节苷脂含量高（248.4 +/- 4.4 μ g，n = 3），几乎完全由N-乙酰神经氨酸组成。皮下生长的肿瘤的神经节苷脂模式是复杂的，而GM 3，GM 2，GM 1和GD 1a是培养的肿瘤细胞中的主要神经节苷脂。这种肿瘤将是一个有用的自然模型，用于评估神经节苷脂和其他糖脂在肿瘤细胞侵袭和转移中的作用。(C)2001年癌症研究运动。
Glycosphingolipid abnormalities have long been implicated in tumour malignancy and metastasis, Gangliosides are a family of sialic acid-containing glycosphingolipids that modulate cell-cell and cell-matrix interactions. Histology and ganglioside composition were examined in a natural brain tumour of the VM mouse strain. The tumour is distinguished from other metastatic turnout models because it arose spontaneously and metastasizes to several organs including brain and spinal cord after subcutaneous inoculation of tumour tissue in the flank. By electron microscopy, the tumour consisted of cells (15 to 20 mum in diameter) that had slightly indented nuclei and scant cytoplasm. The presence of smooth membranes with an absence of junctional complexes was a characteristic ultrastructural feature. No positive immunostaining was found for glial or neuronal markers. The total ganglioside sialic acid content of the subcutaneously grown tumour was low (12.6 +/- 0.9 mug per 100 mg dry wt, n = 6 separate tumours) and about 70% of this was in the form of N-glycolylneuraminic acid. In contrast, the ganglioside content of the cultured VM tumour cells was high (248.4 +/- 4.4 mug, n = 3) and consisted almost exclusively of N-acetylneuraminic acid. The ganglioside pattern of the tumour grown subcutaneously was complex, while GM3, GM2, GM1, and GD1a were the major gangliosides in the cultured tumour cells. This tumour will be a useful natural model for evaluating the role of gangliosides and other glycolipids in tumour cell invasion and metastasis. (C) 2001 Cancer Research Campaign.