GFP Introduction for RAW264.7 Cells by Combining Nanosecond High Voltage Pulse and Millisecond pulse
GFP Introduction for RAW264.7 Cells by Combining Nanosecond High Voltage Pulse and Millisecond pulse
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结合纳秒高压脉冲和毫秒脉冲将 GFP 引入 RAW264.7 细胞
DOI：
10.1109/ppc40517.2021.9733150
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Susumu Kono; Nobuaki Tominaga
中科院分区：
文献类型：
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作者：
茂木雄斗 ; 伊藤暢彦 ; 大田健紘 ; 平栗健史 ; 山本 幹 ; 吉野秀明;河野佑弥，柿ヶ野浩明;Susumu Kono; Nobuaki Tominaga
电穿孔（EP）方法是将遗传物质引入细胞的技术之一。为了提高 EP 方法的效率，开发了一种使用两种不同矩形脉冲组合的脉冲系统。在该系统中，纳秒高压脉冲首先在细胞膜上产生孔隙，然后毫秒脉冲帮助遗传物质进入细胞内部。使用该系统，可以轻松找到人类早幼粒细胞白血病细胞 HL-60 的 GFP（绿色荧光蛋白）转换效率更高的脉冲参数。在本研究中，通过该脉冲系统测试了 RAW264.7（一种源自小鼠细胞的巨噬细胞）的 GFP 引入。
Electroporation (EP) method is one of the techniques used to introduce genetic material into cells. To improve the efficiency of EP method, a pulse system using a combination of two different rectangular pulses were developed. In this system, a nanosecond high voltage pulse creates pores on cell membrane at first, and then a millisecond pulse helps genetic materials move into the inside of cells. Using this system, pulse parameters for the better efficiency of GFP (green fluorescent protein) transition for HL-60, a human promyelocytic leukemia cell, were easily found. In this study, GFP introduction for RAW264.7, a macrophage cell derived from mouse cell, was tested by this pulse system.