Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice
Identification and characterization of NARROW AND ROLLED LEAF 1, a novel gene regulating leaf morphology and plant architecture in rice
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NARROW AND ROLLED LEAF 1（一种调节水稻叶片形态和植物结构的新基因）的鉴定和表征
DOI：
10.1007/s11103-010-9614-7
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发表时间：
2010-06-01
影响因子：
5.1
通讯作者：
Qian, Qian
中科院分区：
文献类型：
--
作者：
Hu, Jiang;Zhu, Li;Qian, Qian
叶片形态是水稻育种的重要农艺性状。我们分离到三个等位基因突变体的窄和卷叶1（nrl 1），表现出减少叶宽和半卷叶的表型和不同程度的矮化。显微镜观察表明，nrl 1 -1突变体与野生型相比，具有较少的纵脉和较小的近轴泡状细胞。NRL 1基因定位于12号染色体，编码纤维素酶样蛋白D4（OsCslD 4）。序列分析表明，在三个等位基因突变体的单碱基取代。OsCslD 4基因的遗传互补和过表达证实了NRL 1的身份。该基因在水稻抽穗期各器官中均有表达，在根、叶鞘、穗等生长旺盛的器官中表达量较高。然而，在突变体叶片中，表达水平低于野生型。我们认为NRL 1编码的OsCslD 4在水稻叶片形态建成和营养生长发育中起着重要作用。
Leaf morphology is an important agronomic trait in rice breeding. We isolated three allelic mutants of NARROW AND ROLLED LEAF 1 (nrl1) which showed phenotypes of reduced leaf width and semi-rolled leaves and different degrees of dwarfism. Microscopic analysis indicated that the nrl1-1 mutant had fewer longitudinal veins and smaller adaxial bulliform cells compared with the wild-type. The NRL1 gene was mapped to the chromosome 12 and encodes the cellulose synthase-like protein D4 (OsCslD4). Sequence analyses revealed single base substitutions in the three allelic mutants. Genetic complementation and over-expression of the OsCslD4 gene confirmed the identity of NRL1. The gene was expressed in all tested organs of rice at the heading stage and expression level was higher in vigorously growing organs, such as roots, sheaths and panicles than in elsewhere. In the mutant leaves, however, the expression level was lower than that in the wild-type. We conclude that OsCslD4 encoded by NRL1 plays a critical role in leaf morphogenesis and vegetative development in rice.