Mitochondrial Creatine Kinase is Decreased in the Serum of Idiopathic Parkinson's Disease Patients
Mitochondrial Creatine Kinase is Decreased in the Serum of Idiopathic Parkinson's Disease Patients
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DOI：
10.14336/ad.2018.0615
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发表时间：
2019-06-01
影响因子：
7.4
通讯作者：
Xu, Yunqi
中科院分区：
文献类型：
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作者：
Xu, Jinghui;Fu, Xiaodi;Xu, Yunqi
线粒体肌酸激酶（MtCK）在线粒体能量代谢过程中起着至关重要的作用。功能障碍与帕金森病（PD）的发病机制有关。因此，我们推测PD患者血清中MtCK活性可能发生改变。然而，迄今为止还没有研究调查这一特定主题，因此我们试图调查PD患者队列中的血清MtCK活性。本研究招募了50名PD患者和30名年龄匹配的对照组。采用免疫抑制法检测血清泛在MtCK （uMtCK）和肉瘤MtCK （sMtCK）活性。探讨PD组血清uMtCK/sMtCK活性与临床特征/参数的相关性。我们的研究显示，与对照组相比，PD组的uMtCK活性显著降低。PD组与对照组血清sMtCK活性差异无统计学意义。PD患者血清uMtCK活性与疾病进展率、病程、发病年龄有显著相关性。而血清uMtCK活性与Hoehn & Yahr分期或主要非运动症状量表之间无显著关系。PD患者血清中uMtCK活性显著降低，这与疾病进展率、病程和发病年龄有关。因此，血清中uMtCK的活性为PD生物标志物的鉴定提供了有用的线索。
Mitochondrial creatine kinase (MtCK) is vital in the process of mitochondrial energy metabolism, and mitochondria! dysfunction has been implicated in the pathogenesis of Parkinson's disease (PD). Therefore, we speculated that MtCK activity could be altered in the serum of PD patients. However, no studies to date have investigated this specific topic, so we sought to investigate the serum MtCK activities among a cohort of PD patients. 50 patients with PD and 30 age-matched controls were recruited for this study. Serum ubiquitous MtCK (uMtCK) and sarcomeric MtCK (sMtCK) activities were assayed using an immunoinhibition method. Correlations between serum uMtCK/sMtCK activities and clinical features/parameters were explored in the PD group. Our study revealed a significant decrease in the uMtCK activity in the PD group when compared with the control group. No significant difference was found in the serum sMtCK activity between the PD and control groups. There was a significant correlation between serum uMtCK activities and the disease progression rate, duration, and age at onset in PD patients. While no significant relationship was found between the serum uMtCK activities and the Hoehn & Yahr stage or main non-motor symptoms scale. There was a significant decrease in the uMtCK activity in the serum of PD patients, which was associated with the rate of disease progression, duration, and age at onset of disease. Therefore, uMtCK activity in serum offers a useful clue for identification of PD biomarkers.