The complete nucleotide sequence of the Pasteurella multocida toxin gene and evidence for a transcriptional repressor, TxaR
The complete nucleotide sequence of the Pasteurella multocida toxin gene and evidence for a transcriptional repressor, TxaR
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多杀性巴氏杆菌毒素基因的完整核苷酸序列和转录抑制因子 TxaR 的证据
DOI：
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发表时间：
1990
影响因子：
3.6
通讯作者：
S. K. Petersen
中科院分区：
文献类型：
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作者：
S. K. Petersen
多杀巴氏杆菌的溶骨毒素在体内和体外诱导骨吸收（Foged et al., 1988; Kimman et al., 1987）。在本报告中，对毒素编码的弓形虫基因进行了测序，并推断出毒素的一级结构显示了一个1285个氨基酸的蛋白质，与金属结合基序具有惊人的同源性。证据表明，在大肠杆菌的转录水平上，弓形虫基因的表达受到抑制。抑制可以通过删除弓形虫上游的一个区域来消除，或者通过假定的同一区域的框架移位突变来消除。在该区域编码的抑制蛋白是高效的反式，被命名为TxaR。
The osteolytic toxin of Pasteurella multocida induces bone resorption in vivo and in vitro (Foged et al., 1988; Kimman et al., 1987). In this report the toxin‐encoding toxA gene is sequenced, and the deduced primary structure of the toxin shows a protein of 1285 amino acids, containing a striking homology to a metal‐binding motif. Evidence that expression of the toxA gene is repressed at a transcriptional level in Escherichia coli is presented. Repression could be abolished either by deletion of a region upstream of toxA, or by a putative frame‐shift mutation in the same region. The repressor protein encoded within this region was efficient in trans, and was named TxaR.