Biomimetic fluorocarbon surfactant polymers reduce platelet adhesion on PTFE/ePTFE surfaces.
Biomimetic fluorocarbon surfactant polymers reduce platelet adhesion on PTFE/ePTFE surfaces.
复制标题
DOI：
10.1163/156856209x426439
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Marchant RE
中科院分区：
文献类型：
--
作者：
Wang S;Gupta AS;Sagnella S;Barendt PM;Kottke-Marchant K;Marchant RE
我们描述了一系列氟碳表面活性剂聚合物，其设计为表面改性剂，用于通过减少血小板粘附来改善ePTFE血管移植物材料的致血栓性。表面活性剂聚合物由具有侧链葡聚糖和全氟十一烷酰基支链的聚（乙烯胺）主链组成。表面改性是通过一个简单的浸涂过程，其中表面活性剂聚合物进行自发的表面诱导吸附和组装的PTFE/ePTFE表面。在动态剪切条件下，在PBS和人全血中，用旋转盘系统检查表面活性剂聚合物在PTFE上的粘附稳定性。在动态流动条件下PTFE/ePTFE表面上血小板粘附的背景下，比较了具有三种不同葡聚糖与全氟化碳比率（1：0.5、1：1和1：2）的氟碳表面活性剂聚合物涂层。在富血小板血浆（PRP）或人全血中，在0-75 dyn/cm 2的剪切应力范围内，所有三种涂层表面均实现了血小板粘附的抑制。有效性取决于表面活性剂聚合物组合物，使得涂覆表面上的血小板粘附随着在0达因/厘米2下氟碳分支密度的增加而显著降低。我们的研究结果表明，氟碳表面活性剂聚合物可以有效地抑制血小板粘附，并证明氟碳表面活性剂聚合物作为ePTFE血管移植物的非血栓形成涂层的潜在应用。
We describe a series of fluorocarbon surfactant polymers designed as surface-modifying agents for improving the thrombogenicity of ePTFE vascular graft materials by the reduction of platelet adhesion. The surfactant polymers consist of a poly(vinyl amine) backbone with pendent dextran and perfluoroundecanoyl branches. Surface modification is accomplished by a simple dip-coating process in which surfactant polymers undergo spontaneous surface-induced adsorption and assembly on PTFE/ePTFE surface. The adhesion stability of the surfactant polymer on PTFE was examined under dynamic shear conditions in PBS and human whole blood with a rotating disk system. Fluorocarbon surfactant polymer coatings with three different dextran to perfluorocarbon ratios (1:0.5, 1:1 and 1:2) were compared in the context of platelet adhesion on PTFE/ePTFE surface under dynamic flow conditions. Suppression of platelet adhesion was achieved for all three coated surfaces over the shear-stress range of 0–75 dyn/cm2 in platelet-rich plasma (PRP) or human whole blood. The effectiveness depended on the surfactant polymer composition such that platelet adhesion on coated surfaces decreased significantly with increasing fluorocarbon branch density at 0 dyn/cm2. Our results suggest that fluorocarbon surfactant polymers can effectively suppress platelet adhesion and demonstrate the potential application of the fluorocarbon surfactant polymers as non-thrombogenic coatings for ePTFE vascular grafts.