Growth of v-src-transformed cells in serum-free medium through the induction of growth factors.
Growth of v-src-transformed cells in serum-free medium through the induction of growth factors.
复制标题
通过生长因子的诱导，v-src 转化细胞在无血清培养基中生长。
DOI：
10.1002/jcp.24303
复制
发表时间：
2013
影响因子：
5.6
通讯作者：
Baserga,Renato
中科院分区：
文献类型：
--
作者：
Deangelis,Tiziana;Quong,Andrew;Morrione,Andrea;Baserga,Renato
The v‐src oncogene is one of only two oncogenes capable of transforming mouse embryo fibroblasts (MEFs) lacking theIGF‐IRgene (R‐cells). R‐/v‐src cells grow robustly in the absence of serum, suggesting the hypothesis that they may produce one or more growth factors that would sustain their ability to proliferate in serum‐free condition. Using proteomic approaches on serum‐free conditioned media derived from v‐src‐transformed cells, we have identified two growth promoting factors: ostepontin and proliferin. Subsequent experiments have indicated that osteopontin plays a prevalent role in promoting growth of v‐src‐transformed cells in serum‐deprived condition. J. Cell. Physiol. 228: 1482–1486, 2013. © 2012 Wiley Periodicals, Inc.
