STRONGYLOIDES-STERCORALIS - IDENTIFICATION OF A PROTEASE THAT FACILITATES PENETRATION OF SKIN BY THE INFECTIVE LARVAE
STRONGYLOIDES-STERCORALIS - IDENTIFICATION OF A PROTEASE THAT FACILITATES PENETRATION OF SKIN BY THE INFECTIVE LARVAE
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DOI：
10.1016/0014-4894(90)90094-s
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发表时间：
1990-02-01
影响因子：
2.1
通讯作者：
NEVA, FA
中科院分区：
文献类型：
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作者：
MCKERROW, JH;BRINDLEY, P;NEVA, FA
宿主入侵和几种蠕虫的组织迁移已被链接到寄生虫衍生的蛋白酶的表达和释放。组织迁移的一个最显著的例子是线虫寄生虫粪类圆线虫的幼虫，它可以以每小时10厘米的速度穿过组织。我们已经证明类圆线虫L3幼虫分泌一种有效的溶组织金属蛋白酶，以促进其快速迁移。这种蛋白酶具有弹性蛋白酶活性，并催化真皮细胞外基质模型的降解。这种酶在类圆线虫病发病机制中的重要性通过金属蛋白酶抑制剂阻止体外皮肤幼虫入侵的观察而得到强调。这些结果证实了蛋白酶在类圆线虫病以及其他相关寄生虫感染中作为毒力因子的作用，并提出了新的治疗方法。
Host invasion and tissue migration of several helminths have been linked to the expression and release of parasite-derived proteases. One of the most remarkable examples of tissue migration is that of larvae of the nematode parasite Strongyloides stercoralis, which can move through tissue at speeds of up to 10 cm per hour. We have shown the Strongyloides L3 larvae secrete a potent histolytic metalloprotease to facilitate their rapid migration. This protease has elastase activity and catalyzes the degradation of a model of dermal extracellular matrix. The importance of this enzyme in the pathogenesis of strongyloidiasis is underscored by the observation that invasion by larvae of skin in vitro is prevented by metalloprotease inhibitors. These results substantiate the role of proteases as virulence factors in strongyloidiasis, as well as other related parasitic infections, and suggest new approaches to therapy.