ECTOPIC EXPRESSION OF THE FLORAL HOMEOTIC GENE AGAMOUS IN TRANSGENIC ARABIDOPSIS PLANTS ALTERS FLORAL ORGAN IDENTITY
ECTOPIC EXPRESSION OF THE FLORAL HOMEOTIC GENE AGAMOUS IN TRANSGENIC ARABIDOPSIS PLANTS ALTERS FLORAL ORGAN IDENTITY
复制标题
DOI：
10.1016/0092-8674(92)90271-d
复制
发表时间：
1992-10-02
期刊：
影响因子：
64.5
通讯作者：
MA, H
中科院分区：
文献类型：
--
作者：
MIZUKAMI, Y;MA, H
拟南芥花同源基因AGAMOUS （AG）是生殖器官（雄蕊和心皮）发育所必需的基因。在ag突变体中，ag功能的丧失导致这些器官转化为花被器官（花瓣和萼片）。相反，另一个花同源基因aptala2 （AP2）的突变导致花被器官被生殖器官取代。在这些观察的基础上，有人提出AG和AP2以拮抗方式起作用。为了验证这一假设，我们研究了异位表达AG对转基因拟南芥植株的影响。转基因植株的花表现出一系列与ap2突变体相似的表型。这些实验为AG与AP2功能之间的拮抗作用提供了直接证据，结果强烈表明AG确实抑制AP2功能。
The Arabidopsis floral homeotic gene AGAMOUS (AG) is required for development of the reproductive organs (stamens and carpels). In ag mutants, the loss of AG function leads to the conversion of these organs to the perianth organs (petals and sepals). In contrast, mutations in another floral homeotic gene, APETALA2 (AP2), result in the replacement of the perianth organs by the reproductive organs. On the basis of these observations, it has been proposed that AG and AP2 act in an antagonistic fashion. To test this hypothesis, we have studied the effects of ectopically expressed AG in transgenic Arabidopsis plants. The flowers of the transgenic plants exhibit a range of phenotypes mirroring those of ap2 mutants. These experiments provide direct evidence of the proposed antagonism between AG and AP2 functions, and the results strongly suggest that AG does indeed inhibit AP2 function.