Cell sheet engineering: Recreating tissues without biodegradable scaffolds
Cell sheet engineering: Recreating tissues without biodegradable scaffolds
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DOI：
10.1016/j.biomaterials.2005.04.061
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发表时间：
2005-11-01
期刊：
影响因子：
14
通讯作者：
Okano, T
中科院分区：
文献类型：
--
作者：
Yang, J;Yamato, M;Okano, T
虽然组织工程一直被认为具有巨大的潜力，但使用可生物降解支架的传统应用限制了该领域的进展，表明需要新的方法。为了避免传统的组织工程方法及其相关缺点，我们之前已经开发了使用温度响应培养皿的细胞片工程。使用温度敏感的培养皿，培养的细胞可以通过简单的温度变化作为完整的薄片收获，从而避免使用蛋白质水解酶。因此，细胞片工程允许通过直接将细胞片移植到宿主组织或通过单个细胞片分层创建三维结构来实现组织再生。通过避免使用任何额外的材料，如载体基质或支架，可以避免与传统组织工程方法相关的并发症，如宿主对植入聚合物材料的炎症反应。因此，细胞片工程呈现出几个显著的优势，并且可以克服以前限制生物可降解支架组织工程的许多问题。(c) 2005 Elsevier Ltd版权所有。
While tissue engineering has long been thought to possess enormous potential, conventional applications using biodegradable scaffolds have limited the field's progress, demonstrating a need for new methods. We have previously developed cell sheet engineering using temperature-responsive culture dishes in order to avoid traditional tissue engineering approaches, and their related shortcomings. Using temperature-responsive dishes, cultured cells can be harvested as intact sheets by simple temperature changes, thereby avoiding the use of proteolytic enzymes. Cell sheet engineering therefore allows for tissue regeneration by either direct transplantation of cell sheets to host tissues or the creation of three-dimensional structures via the layering of individual cell sheets. By avoiding the use of any additional materials such as carrier substrates or scaffolds, the complications associated with traditional tissue engineering approaches such as host inflammatory responses to implanted polymer materials, can be avoided. Cell sheet engineering thus presents several significant advantages and can overcome many of the problems that have previously restricted tissue engineering with biodegradable scaffolds. (c) 2005 Elsevier Ltd. All rights reserved.