Fractalkine receptor CX(3)CR1 is expressed in epithelial ovarian carcinoma cells and required for motility and adhesion to peritoneal mesothelial cells.
Fractalkine receptor CX(3)CR1 is expressed in epithelial ovarian carcinoma cells and required for motility and adhesion to peritoneal mesothelial cells.
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DOI：
10.1158/1541-7786.mcr-11-0256
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发表时间：
2012-01
期刊：
影响因子：
--
通讯作者：
Barbolina MV
中科院分区：
文献类型：
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作者：
Kim M;Rooper L;Xie J;Kajdacsy-Balla AA;Barbolina MV
上皮性卵巢癌是一种致命的疾病，其转移进展的机制知之甚少。使用人类标本和已建立的细胞系，我们确定G蛋白偶联的七跨膜fractalkine受体（CX 3CR 1）在原发性和转移性卵巢癌细胞中表达。卵巢癌细胞以CX 3CR 1依赖性方式向CX 3CL 1（CX 3CR 1的特异性配体）迁移。CX 3CR 1的沉默使向人卵巢癌腹水的迁移减少了约70%。重要的是，卵巢癌细胞与人腹膜间皮细胞的粘附依赖于CX 3CL 1/CX 3CR 1信号传导。此外，CX 3CL 1能够诱导细胞增殖。总之，我们的数据表明，fractalkine网络可能是上皮性卵巢癌进展的主要贡献者，并且需要进一步关注其在这种致命恶性肿瘤转移中的作用。
Epithelial ovarian carcinoma is a deadly disease, and little is known about the mechanisms underlying its metastatic progression. Using human specimens and established cell lines, we determined that the G protein-coupled seven-transmembrane fractalkine receptor (CX3CR1) is expressed in primary and metastatic ovarian carcinoma cells. Ovarian carcinoma cells robustly migrated toward CX3CL1, a specific ligand of CX3CR1, in a CX3CR1-dependent manner. Silencing of CX3CR1 reduced migration toward human ovarian carcinoma ascites fluid by approximately 70%. Importantly, adhesion of ovarian carcinoma cells to human peritoneal mesothelial cells was dependent on CX3CL1/CX3CR1 signaling. In addition, CX3CL1 was able to induce cellular proliferation. Together, our data suggest that the fractalkine network may function as a major contributor to the progression of epithelial ovarian carcinoma, and further attention to its role in the metastasis of this deadly malignancy is warranted.