Failure of 1,25 Dihydroxycholecalciferol [1,25-(OH)1-D3] to Modify Cyclic AMP Levels in Parathyroid Hormone-Treated and Untreated Bone Cells (1)
Failure of 1,25 Dihydroxycholecalciferol [1,25-(OH)1-D3] to Modify Cyclic AMP Levels in Parathyroid Hormone-Treated and Untreated Bone Cells (1)
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1,25 二羟基胆钙化醇 [1,25-(OH)1-D3] 无法改变甲状旁腺激素处理和未处理的骨细胞中的环 AMP 水平 (1)
DOI：
10.3109/07435807609052930
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发表时间：
1976
期刊：
影响因子：
1.9
通讯作者：
I. Dowling
中科院分区：
文献类型：
--
作者：
W. A. Peck;I. Dowling
用1，25-(OH)2-D3(1 ng/ml-25 ng/ml)处理2.5min。至60分钟。不改变去骨膜大鼠颅骨分离的骨细胞或大鼠骨膜组织分离的细胞的环磷酸腺苷水平。1，25-(OH)2-D3不能拮抗甲状旁腺素(10 ng/ml-1 ug/ml)引起的环磷酸腺苷(CAMP)的急剧升高。在其他体系中具有生物活性的两个单独的1，25-(OH)2-D3制剂在各种实验条件下都是无效的。这些结果表明，1，25-(OH)2-D3在PTH处理和未处理的骨细胞中不是环磷酸腺苷代谢的急性调节剂。
Treatment with 1,25-(OH)2-D3 (1 ng/ml - 25 ng/ml) for periods ranging from 2.5 min. to 60 min. did not alter cyclic AMP levels in bone cells isolated from periosteum-free rat calvaria, or in cells isolated from rat periosteal tissues. 1,25-(OH)2-D3 failed to modify the acute increases in cyclic AMP elicited by PTH (10 ng/ml -1 ug/ml). Two separate 1,25-(OH)2-D3 preparations, biologically active in other systems, were ineffective under a wide variety of experimental conditions. These results suggest that 1,25-(OH)2-D3 is not an acute modulator of cyclic AMP metabolism in PTH-treated and untreated bone cells.