A honeycomb collagen carrier for cell culture as a tissue engineering scaffold
A honeycomb collagen carrier for cell culture as a tissue engineering scaffold
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DOI：
10.1046/j.1525-1594.2001.025003213.x
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发表时间：
2001-03-01
影响因子：
2.4
通讯作者：
Miyata, T
中科院分区：
文献类型：
--
作者：
Itoh, H;Aso, Y;Miyata, T
作为用于细胞培养的三维载体，蜂窝结构细胞支架由I型、II型和III型端肽胶原蛋白产生。蜂窝孔的直径范围为100至1，000 μ m。孔的深度为10至3,000 mm。支架具有弹性和硬度。创建各种形状很容易，并且这些形状很容易保持。将人成纤维细胞、CHO-K1、BHK-21和牛内皮细胞与支架一起培养。这些细胞的生长曲线令人满意。这些结果表明，这种载体是一种合适的细胞培养支架，并将是有用的三维组织工程支架。
As a three-dimensional carrier for cell culture, a honeycomb structure cell scaffold was created from atelopeptide collagen Types I, II, and III. The diameter of the honeycomb pores ranged from 100 to 1,000 mum. The depth of the pores was from 10 to 3,000 mm. The scaffold was elastic and hard. Creation of various shapes was easy, and these shapes were easily maintained. Human fibroblasts, CHO-K1, BHK-21, and bovine endothelial cells were cultured with the scaffold. The growth curves of these cells were satisfactory. These results suggest that this carrier is a suitable scaffold for cell culture and will be useful as a three-dimensional tissue engineering scaffold.