Localization of occludin, ZO-1, and pan-cadherin in rabbit ciliary epithelium and iris vascular endothelium.
Localization of occludin, ZO-1, and pan-cadherin in rabbit ciliary epithelium and iris vascular endothelium.
复制标题
occludin、ZO-1 和泛钙粘蛋白在兔睫状上皮和虹膜血管内皮中的定位。
DOI：
10.1007/s004180000195
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Freddo,TF
中科院分区：
文献类型：
--
作者：
Wu,P;Gong,H;Richman,R;Freddo,TF
以前的研究已经使用传统的电子显微镜和冷冻骨折来鉴定哺乳动物眼睛中血水屏障的形态等同物。这些等同物是相邻的非色素纤毛上皮细胞之间的紧密连接和虹膜血管内皮细胞之间的紧密连接，构成了顶端外侧连接复合体的一部分。最近的研究已经开始揭示紧密连接的分子组装，并发现了一些变异性。在本研究中，我们的目的是探测兔眼的睫状上皮和虹膜血管内皮，以确定与其他组织紧密连接相关的某些分子成分是否也作为血水屏障的一部分存在。选择的成分是occludin， ZO-1和具有代表性的粘附连接相关钙粘蛋白。采用免疫组织化学和免疫电镜方法。结果表明，occludin只分布在已知的紧密连接部位。ZO-1也在这些位置表达，但它的分布超出了occludin的范围，沿着邻近的膜延伸。泛钙粘蛋白在睫状上皮内普遍表达，在虹膜血管内皮中表达不明显。结果表明，occludin和ZO-1是正常兔眼血水屏障的组成部分。
Previous studies have used conventional electron microscopy and freeze fracture to identify the morphological equivalents of the blood–aqueous barrier in the mammalian eye. These equivalents are the tight junctions that form a part of the apicolateral junctional complex between adjacent non-pigmented ciliary epithelial cells and the tight junctions present between endothelial cells of the iris vasculature. Recent investigations have begun to unravel the molecular assembly of the tight junction and some variability has been found. Our goal in the present study was to probe the ciliary epithelium and iris vascular endothelium of the rabbit eye to determine if certain molecular constituents associated with tight junctions in other tissues are also present as parts of the blood–aqueous barrier. The selected constituents were occludin, ZO-1, and a representative, adherens junction-related cadherin. Immunohistochemical and immunoelectron microscopic methods were used. The results showed that occludin was distributed exclusively at known locations of tight junctions. ZO-1 was also expressed at these locations but its distribution extended beyond that of occludin, along the adjacent membranes. Pan-cadherin was expressed ubiquitously within the ciliary epithelium and negligibly in iris vascular endothelium. Our results demonstrate that occludin and ZO-1 are integral components of the blood–aqueous barrier of the normal rabbit eye.