Simultaneous removal of nitrogen and phosphorous by heterotrophic nitrification-aerobic denitrification of a metal resistant bacterium Pseudomonas putida strain NP5
Simultaneous removal of nitrogen and phosphorous by heterotrophic nitrification-aerobic denitrification of a metal resistant bacterium Pseudomonas putida strain NP5
复制标题
耐金属恶臭假单胞菌NP5异养硝化-好氧反硝化同时脱氮除磷
DOI：
10.1016/j.biortech.2019.121360
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发表时间：
2019-08-01
影响因子：
11.4
通讯作者：
Wang, Rui-Hua
中科院分区：
文献类型：
--
作者：
Yang, Lei;Wang, Xu-Hui;Wang, Rui-Hua
分离到一株具有高效异养硝化、好氧反硝化和聚磷能力的新菌株NP5，经鉴定为恶臭假单胞菌NP5。去除的氨氮和磷酸盐主要通过同化作用转化为胞内成分，异养硝化过程中硝化中间产物和N2O的积累很少。最佳脱氮条件为：以琥珀酸为碳源，C/N为10，P/N为0.2，温度30℃，盐度0%，摇床转速160rpm。此外，菌株NP5对重金属和纳米颗粒具有极强的抗性。在所测试的金属中，Cr6+的毒性最强，并且可以同时去除。此外，厌氧条件下有明显的释磷现象，反复暴露在厌氧/好氧条件下可以显著提高营养盐的去除效果。此外，脱氮除磷关键酶的成功表达为同步硝化、反硝化和除磷的可能性提供了额外的证据。
A novel strain NP5 with efficient heterotrophic nitrification, aerobic denitrification and phosphorus accumulation ability was isolated and identified as Pseudomonas putida strain NP5. The removed ammonium and phosphate were mainly converted into intracellular components by assimilation, and negligible nitrification intermediates and N2O were accumulated during heterotrophic nitrification. In addition, the optimal conditions for nutrient removal were: succinate as carbon source, C/N 10, P/N 0.2, temperature 30 degrees C, salinity 0% and shaking speed 160 rpm. Besides, strain NP5 possessed an exceptional heavy metal and nanoparticles resistance. Cr6+ was found to be the most toxic among the tested metals, and it could be removed simultaneously. Moreover, an obvious phosphorus release was observed under anaerobic condition, and repeated exposure to the anaerobic/aerobic conditions could significantly improve the nutrient removal. Furthermore, the successful expression of key enzymes for nitrogen and phosphorous removal provided additional evidence for possibility of simultaneous nitrification, denitrification and phosphorus removal.