A NEW MEMBER OF THE LEUCINE ZIPPER CLASS OF PROTEINS THAT BINDS TO THE HLA DR-ALPHA PROMOTER
A NEW MEMBER OF THE LEUCINE ZIPPER CLASS OF PROTEINS THAT BINDS TO THE HLA DR-ALPHA PROMOTER
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DOI：
10.1126/science.2321018
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发表时间：
1990-03-30
期刊：
影响因子：
56.9
通讯作者：
GLIMCHER, LH
中科院分区：
文献类型：
--
作者：
LIOU, HC;BOOTHBY, MR;GLIMCHER, LH
从转化的人B细胞系中得出的几个突变体在表达主要的组织相容性复合物（MHC）II类基因方面有缺陷。在至少一个这样的突变线中未能表达II类基因的未能映射到MHC II级X框，这是启动子区域中保守的转录元件。编码DNA结合蛋白的互补DNA（人X盒结合蛋白，HXBP-1），其靶标是人类Dr.Alpha。 X盒子和3''侧面区域现已克隆。互补的DNA编码了与C-Jun原始癌基产物具有结构相似性的蛋白质，其靶序列与C-Jun的腔圆底靶靶序列密切相关。 HXBP-1 DNA靶序列的突变降低了Alpha博士。体内启动子活性。这些研究表明，HXBP-1蛋白在B细胞中充当转录因子。
Several mutants derived from transformed human B cell lines are defective in expressing major histocompatibility complex (MHC) class II genes. The failure to express a class II gene in at least one such mutant line has been mapped to the MHC class II X box, a conserved transcriptional element in the promoter region. A complementary DNA encoding a DNA-binding protein (human X box binding protien, hXBP-1) whose target is the human DR.alpha. X box and the 3'' flanking region has now been cloned. The complementary DNA encoded a protein with structural similarities to the c-jun proto-oncogene product, and its target sequence was closely related to the palindromic target sequence of c-jun. Mutation of the hXBP-1 DNA target sequence decreased DR.alpha. promoter activity in vivo. These studies suggest that the hXBP-1 protein acts as a transcription factor in B cells.