Antiproliferative activity of REIC/Dkk-3 and its significant down-regulation in non-small-cell lung carcinomas
Antiproliferative activity of REIC/Dkk-3 and its significant down-regulation in non-small-cell lung carcinomas
复制标题
DOI：
10.1006/bbrc.2001.5972
复制
发表时间：
2001-11-23
影响因子：
3.1
通讯作者：
Namba, M
中科院分区：
文献类型：
--
作者：
Tsuji, T;Nozaki, I;Namba, M
我们最近报道了REIC/Dkk-3基因的克隆，该基因的表达在许多人永生化细胞系和肿瘤衍生细胞系中被证明是下调的[T. Tsuji等人（2000）Biochem.Biophys.通信资源268，20-24]。在本研究中，我们证明了外源性REIC/Dkk-3基因在肿瘤细胞中的表达抑制细胞生长。此外，REIC/Dkk-3 mRNA在正常人细胞中的水平在细胞周期的晚期G1期最低。我们发现REIC/Dkk-3在手术切除的非小细胞肺癌中表达显著下调。我们确定了REIC/Dkk-3基因座的染色体11 p15，杂合性丢失已被频繁地观察到在人类肿瘤。这些发现表明REIC/Dkk-3可能具有肿瘤抑制作用。(C)北京：科学出版社.
We recently reported the cloning of the REIC/Dkk-3 gene, whose expression was shown to be downregulated in many human immortalized and tumor-derived cell lines [T. Tsuji et al. (2000) Biochem. Biophys. Res. Commun. 268, 20-24]. In the present study, we demonstrated that expression of the exogenous REIC/Dkk-3 gene in tumor cells inhibited cell growth. Furthermore, the level of REIC/Dkk-3 mRNA in normal human cells was lowest in the late G, phase during the cell cycle. Then we found that the expression of REIC/Dkk-3 was significantly down-regulated in surgically resected non-small-cell lung carcinomas. We determined the REIC/Dkk-3 locus on chromosome 11p15, where loss of heterozygosity has frequently been observed in human tumors. These findings indicate that REIC/Dkk-3 may function as a tumor suppressor. (C) 2001 Academic Press.