Cultivation of Cells in a Physiological Plasmax Medium Increases Mitochondrial Respiratory Capacity and Reduces Replication Levels of RNA Viruses.
Cultivation of Cells in a Physiological Plasmax Medium Increases Mitochondrial Respiratory Capacity and Reduces Replication Levels of RNA Viruses.
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DOI：
10.3390/antiox11010097
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发表时间：
2021-12-30
期刊：
影响因子：
--
通讯作者：
Ivanov AV
中科院分区：
文献类型：
--
作者：
Golikov MV;Karpenko IL;Lipatova AV;Ivanova ON;Fedyakina IT;Larichev VF;Zakirova NF;Leonova OG;Popenko VI;Bartosch B;Kochetkov SN;Smirnova OA;Ivanov AV
代谢途径的改变通常与各种病理的发展有关，包括癌症、炎症性疾病、肥胖和代谢综合征。识别特定代谢事件的失调可能产生药物干预的策略。然而，由于使用的经典细胞培养液不能反映血浆中存在的代谢物成分，并且会导致培养细胞的非生理性适应，这类研究受到了阻碍。近年来，有两个小组提出了旨在反映人血浆成分的介质，即人血浆样介质(HPLM)和血浆最大值(Plamax)。在这里，我们描述了在四种不同的哺乳动物细胞系中，血浆最大增强线粒体呼吸作用。这与庞大的线粒体网络的形成和活性氧物种(ROS)的产生增加有关。有趣的是，在胞浆中培养的细胞显示的溶酶体明显少于使用任何标准培养基时的溶酶体。最后，在胞浆中培养的细胞支持各种RNA病毒的复制，例如丙型肝炎病毒(丙型肝炎病毒)、甲型流感病毒(IAV)、严重急性呼吸综合征相关冠状病毒2(SARS-CoV-2)和其他几种病毒，尽管水平较低且动力学延迟。总之，病毒感染背景下的新陈代谢研究，特别是关于线粒体、溶酶体或氧化还原系统的研究，应该在血浆最大培养基中进行。
Changes in metabolic pathways are often associated with the development of various pathologies including cancer, inflammatory diseases, obesity and metabolic syndrome. Identification of the particular metabolic events that are dysregulated may yield strategies for pharmacologic intervention. However, such studies are hampered by the use of classic cell media that do not reflect the metabolite composition that exists in blood plasma and which cause non-physiological adaptations in cultured cells. In recent years two groups presented media that aim to reflect the composition of human plasma, namely human plasma-like medium (HPLM) and Plasmax. Here we describe that, in four different mammalian cell lines, Plasmax enhances mitochondrial respiration. This is associated with the formation of vast mitochondrial networks and enhanced production of reactive oxygen species (ROS). Interestingly, cells cultivated in Plasmax displayed significantly less lysosomes than when any standard media were used. Finally, cells cultivated in Plasmax support replication of various RNA viruses, such as hepatitis C virus (HCV) influenza A virus (IAV), severe acute respiratory syndrome-related coronavirus 2 (SARS-CoV-2) and several others, albeit at lower levels and with delayed kinetics. In conclusion, studies of metabolism in the context of viral infections, especially those concerning mitochondria, lysosomes, or redox systems, should be performed in Plasmax medium.
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癌细胞对葡萄糖限制和双胍类药物敏感性的代谢决定因素。
影响因子：
64.8
作者：
Birsoy, Kivanc;Possemato, Richard;Lorbeer, Franziska K.;Bayraktar, Erol C.;Thiru, Prathapan;Yucel, Burcu;Wang, Tim;Chen, Walter W.;Clish, Clary B.;Sabatini, David M.
通讯作者：
Sabatini, David M.
影响因子：
4.6
作者：
Averous J;Lambert-Langlais S;Mesclon F;Carraro V;Parry L;Jousse C;Bruhat A;Maurin AC;Pierre P;Proud CG;Fafournoux P
通讯作者：
Fafournoux P
影响因子：
3.7
作者：
Ivanov AV;Smirnova OA;Ivanova ON;Masalova OV;Kochetkov SN;Isaguliants MG
通讯作者：
Isaguliants MG
影响因子：
5.6
作者：
Chalecka M;Kazberuk A;Palka J;Surazynski A
通讯作者：
Surazynski A
影响因子：
29
作者：
Codo, Ana Campos;Davanzo, Gustavo Gastao;Moraes-Vieira, Pedro M.
通讯作者：
Moraes-Vieira, Pedro M.