Locations of the motor endplate band and motoneurons innervating the sternomastoid muscle in the rat.
Locations of the motor endplate band and motoneurons innervating the sternomastoid muscle in the rat.
复制标题
大鼠运动终板带和支配胸骨乳突肌的运动神经元的位置。
DOI：
10.1002/ar.21312
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Sobotka,Stanislaw
中科院分区：
文献类型：
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作者：
Zhang,Xiaolin;Mu,Liancai;Su,Hungxi;Sobotka,Stanislaw
胸锁乳突肌（SCM）是一块长肌肉，有两个腹部，即位于颈部外侧的胸锁乳突肌（SM）和锁乳突肌（CM）。它已被广泛用作肌肉和肌皮瓣，用于重建口腔和面部缺陷，并作为神经再生研究的候选者。因此，SCM 的精确神经解剖学对于指导神经再生手术至关重要。在这项研究中，研究人员对大鼠 SM 进行了研究，使用全乙酰胆碱酯酶 (AChE) 染色记录运动终板 (MEP) 的带状模式，并使用逆行辣根过氧化物酶 (HRP) 示踪技术确定支配肌肉的运动神经元的位置。结果表明，SM 和 CM 中的 MEP 组织成一条带，位于肌肉的中部。将 HRP 注射到 SM 的 MEP 带后，在尾部延髓 (MO)、C1 和 C2 中鉴定出同侧标记的运动神经元。发现 SM 运动神经元在 MO 下部和 C1 背内侧 (DM) 核中形成单列。相比之下，C2 中标记的 SM 运动神经元形成一个（DM 核）、两个 [DM 和腹外侧 (VL) 核] 或三个 [DM、VL 和腹内侧 (VM)] 列。这些发现不仅对于理解肌肉的神经控制很重要，而且对于评估选择 SM 作为目标肌肉时给定神经支配手术的成功率也很重要。 Anat Rec，2011。© 2010 Wiley-Liss, Inc.
Sternocleidomastoid (SCM) is a long muscle with two bellies, sternomastoid (SM) and cleidomastoid (CM) in the lateral side of the neck. It has been widely used as muscle and myocutaneous flap for reconstruction of oral cavity and facial defects and as a candidate for reinnervation studies. Therefore, exact neuroanatomy of the SCM is critical for guiding reinnervation procedures. In this study, SM in rats were investigated to document banding pattern of motor endplates (MEPs) using whole‐mount acetylcholinesterase (AChE) staining and to determine locations of the motoneurons innervating the muscle using retrograde horseradish peroxidase (HRP) tracing technique. The results showed that the MEPs in the SM and CM were organized into a single band which was located in the middle portion of the muscle. After HRP injections into the MEP band of the SM, ipsilaterally labeled motoneurons were identified in the caudal medulla oblongata (MO), C1, and C2. The SM motoneurons were found to form a single column in lower MO and dorsomedial (DM) nucleus in C1. In contrast, the labeled SM motoneurons in C2 formed either one (DM nucleus), two [DM and ventrolateral (VL) nuclei], or three [DM, VL, and ventromedial (VM)] columns. These findings are important not only for understanding the neural control of the muscle but also for evaluating the success rate of a given reinnervation procedure when the SM is chosen as a target muscle. Anat Rec, 2011. © 2010 Wiley‐Liss, Inc.